| 香石竹ACC氧化酶基因的克隆及其正义反义表达载体的构建 |
| |
| 引用本文： | 李涵,张婷,张颢,李树发,姚有林,于丽霞,鄢波.香石竹ACC氧化酶基因的克隆及其正义反义表达载体的构建[J].北方园艺,2007(11):177-179. |
| |
| 作者姓名： | 李涵 张婷 张颢 李树发 姚有林 于丽霞 鄢波 |
| |
| 作者单位： | 1. 云南省农业科学院,花卉研究所,云南,昆明,650205
2. 西南林学院,植物保护学院,云南,昆明,650205 |
| |
| 基金项目： | 云南省农业科技攻关计划 |
| |
| 摘 要： | 利用在Genebank上已报道的香石竹ACC氧化酶(ACO)基因的CDNA序列设计特异引物,以香石竹品种'MASTER'的eDNA为模板进行PCR扩增,克隆香石竹ACC氧化酶(AC0)基因.测序结果显示,克隆基因的序列与报道序列完全一致.将克隆的ACC氧化酶(AC0)基因分别连接到植物表达载体PBI121启动子CaMV 35S的上游及下游,构建香石竹ACC氧化酶(Aco)基因的正义表达栽体PBI121-ACO及反义表达载体PBI121-anti ACO.经PCR鉴定,基因已成功构建到表达裁体上.为香石竹抗衰老基因工程育种奠定了基础.
|
| 关 键 词： | 香石竹 AOC氧化酶基因 正义反义表达载体 |
| 文章编号： | 1001-0009（2007）11-0177-03 |
| 收稿时间： | 2007-06-08 |
| 修稿时间： | 2007年6月8日 |
ACC Oxidase Gene Clonging in Carnation and the Construction of Its' Plant Expression Vectors and Antisense Expression Vectors |
| |
| LI Han,ZHANG Ting,ZHANG Hao,LI Shu-fa,YAO You-lin,YU Li-xia,YAN Bo.ACC Oxidase Gene Clonging in Carnation and the Construction of Its'''' Plant Expression Vectors and Antisense Expression Vectors[J].Northern Horticulture,2007(11):177-179. |
| |
| Authors: | LI Han ZHANG Ting ZHANG Hao LI Shu-fa YAO You-lin YU Li-xia YAN Bo |
| |
| Institution: | 1. Flower Research lnsistute of Yunnan Academy of Agriculture Scienee,Kunming,650205 ,China; 2. Plant Protection College,West-south Forestry University, Yunan,kunming,650205 ,China |
| |
| Abstract: | Basing on the cDNA sequence of ACC Oxidase Gene from carnation in Genebank,we designed a pair of special primers,according to PCR,amplified ACC Oxidase Gene of carnation using the cDNA of MASTER' as template.The sequence analysis showed that the cloned sequence was accord with the reported totally,PCR product was cloned into the upstream and downstream of CaMV 35S promoter in plant expression vector-PBI121 plasmid separately,established expression vectors(PBI121-ACO) and antisense expression vectors(PBI121-antiACO).PCR identification showed gene had been cloned into expression vector successfully which can be used for old resistant breeding of gene engineering. |
| |
| Keywords: | Carnation ACC Oxidase Gene Expression and antisense expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
