| 可控环境下光照时间对铁皮石斛组培苗生长发育的影响 |
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| 引用本文： | 鲍顺淑,贺冬仙,郭顺星.可控环境下光照时间对铁皮石斛组培苗生长发育的影响[J].中国农业科技导报,2007,9(6):90-94. |
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| 作者姓名： | 鲍顺淑 贺冬仙 郭顺星 |
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| 作者单位： | [1]中国农业大学农业部设施农业生物环境工程重点开放实验室,北京100083 [2]中国医学科学院中国协和医科大学药用植物研究所,北京100094 |
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| 基金项目： | 农业部设施农业生物环境工程学科建设基金 |
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| 摘 要： | 在人工光型密闭式植物工厂的可控环境条件下，光照时间会影响铁皮石斛（Dendrobium officinale）组培苗的生长发育。将鲜重约为300mg的铁皮百斛单腋芽作为外植体在温度22±1℃、湿度65±5％、光照强度68±9μmol·m^-2·s^-2。、光期的CO2浓度800±50μmol·mol^-1。的环境条件下，设置光照时间为6h·d^-1、9h·d^-1、12h·d^-1、15h·d^-1、18h·d^-1的5组试验区进行培育。该可控环境下培育92d后，光照时问为12h·d^-1的试验区的铁皮石斛组培苗的净光合速率和叶绿素含量较高，干重和腋芽数增加较多，表现出良好的生长与繁殖能力。因此，人工光型密闭式植物工厂内铁皮石斛组培苗的适宜光照周期为12h·d^-1。
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| 关 键 词： | 叶绿素含量 可控环境 光合速率 多糖含量 |
| 文章编号： | 1008-0864(2007)06-0090-05 |
| 收稿时间： | 2007-08-28 |
| 修稿时间： | 2007年8月28日 |
Suitable Photoperiod of Dendrobium Officinale in |vitro under Controlled Environment |
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| BAO Shun-shu,HE Dong-xian,GUO Shun-xing.Suitable Photoperiod of Dendrobium Officinale in |vitro under Controlled Environment[J].Journal of Agricultural Science and Technology,2007,9(6):90-94. |
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| Authors: | BAO Shun-shu HE Dong-xian GUO Shun-xing |
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| Institution: | 1. Key Lab of Agricultural Bio-environment Engineering of Ministry of Agriculture, China Agricultural University, Beijing 100083 |2. Institute of Medicinal Plant Development|Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100094,China |
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| Abstract: | The effect of photoperiod on growth and development of Dendrobium officinale in vitro was studied under controlled environment in a closed plant factory with artificial lighting. Single explant each with 300 mg fresh mass of D. officinale were planted in five treatments with photoperiods of 6, 9, 12, 15, and 18h·d^-1 under controlled environment at air temperature of 22 ± 1℃, relative humidity of 65 ± 5%, lighting intensity of 68 ± 9 μmol · m^^-1· s^-1, and COz concentration of 800 ± 50 μmol· mol -l in photoperiock The net photosynthetic rate, chlorophyll content, dry mass, and buds in treatment of photoperiod of 12 h·d^-1were higher than that in other treatments, and resulted in greater growth and reproduction for D. officinale producction in vitro after the explants were cultured 92 days. Therefore, the photoperiod of 12 h·d^-1 for D. officinale production in vitro is suitable in tthe closed plant factory with artificial lighting. |
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| Keywords: | chlorophyll content controlled environment photosynthetic rate polysaccharide content |
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