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兰州地区宠物犬弓形虫病血清学检测分析
引用本文:郑红星,叶得河,祁珊珊.兰州地区宠物犬弓形虫病血清学检测分析[J].中国畜牧兽医,2010,37(11):172-173.
作者姓名:郑红星  叶得河  祁珊珊
作者单位:(1.甘肃畜牧工程职业技术学院兽医系,武威 733006; 2.甘肃农业大学动物医学院,兰州 730070)
摘    要:为了解兰州地区宠物犬弓形虫病的感染情况以及不同弓形虫检测方法检测结果的差异,本研究对兰州地区272例宠物诊所受检犬进行弓形虫阳性检出率的统计分析;对66份犬血样进行间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测,并将2种方法进行比较。结果表明,弓形虫的感染率与年龄有关,随着年龄的增长感染率增加,犬小于1岁感染率最低,为3.77%,1~3岁感染率为10.59%,3~5岁感染率为15.28%,大于5岁时感染率最高,为22.58%,不同性别之间感染无显著差异(P>0.05);IHA与ELISA检测结果经t检验比较差异不显著(P>0.05)。

关 键 词:兰州  宠物犬  弓形虫  血清学  检测  

Serological Detection and Analysis of Toxoplasma gondii Infection in Dogs in Lanzhou
ZHENG Hong-xing,YE De-he,QI Shan-shan.Serological Detection and Analysis of Toxoplasma gondii Infection in Dogs in Lanzhou[J].China Animal Husbandry & Veterinary Medicine,2010,37(11):172-173.
Authors:ZHENG Hong-xing  YE De-he  QI Shan-shan
Institution:(1.Department of Veterinary Medicine, Gansu Polytechnic College of Animal Husbandry and Engineering,Wuwei 733006, China; 2.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China)
Abstract:In order to find the infection information of Toxoplasma gondii infection in dogs in Lanzhou and the different detection results in different test methods. 272 dogs from veterinary surgeons were detected to find the masccline of Toxoplasma and the detection rates were statistical analysed; 66 pieces of blood sample were detected by indirect hemagglutination test and enzyme-linked immunosorbent test, the two methods were compared. The results showed that there was a relationship between the infection of Toxoplasma and the age of dogs, the infection rats increased corresponding to the increasing of age, the infection rate was 3.77% in less than one years old dogs, 10.59% in one to three years dogs, 15.28% in three to five years dogs, 22.58% in more than five years dogs. There were no significant deviation in different gender(P>0.05); there was no significant deviation in detection results of HA and ELISA through t test comparison(P>0.05). The method of IHA was applied to epidemiological study in large scale.
Keywords:Lanzhou  dogs  Toxoplasma gondii  serology  detection
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