Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans,animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans,animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis

Authors:	Noriko IDO Kaori IWABUCHI Yusuke SATO’O Yasuo SATO Masaru SUGAWARA Gakuji YAEGASHI Masaru KONNO Masato AKIBA Kiyoshi TANAKA Katsuhiko OMOE Ikuo UCHIDA

Abstract:	Fifty-one Salmonella enterica serovar 4,5],12:i:- (S. 4, 5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson’s diversity indices DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,5],12:i:- suggested the existence of the links between human infections and animal reservoirs.

Keywords:	MLVA monophasic variant PFGE Salmonella 4 [5] 12:i:-(S 4 [5] 12:i:-) S Typhimurium