Influence of different methods of collection from the canine epididymides on
post-thaw caudal epididymal sperm quality |
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| Authors: | Tatsuya HORI Tetsuya ATAGO Masanori KOBAYASHI Eiichi KAWAKAMI |
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| Institution: | 1)Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan |
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| Abstract: | Canine epididymal sperm was collected from the cauda epididymis using 2
different methods (flushing and mincing) to compare the qualities (the percentage of
progressively motile, viable, morphologically abnormal, immature and intact acrosomes)
before and after freezing and thawing. No significant difference was noted in the quality
of the cauda epididymal sperm immediately after collection and after freezing-thawing
between the collection methods, although the mean levels of sperm quality with the
flushing method were slightly better than that of the mincing method. The flushing method
is simple and free of blood contamination, although the vas deferens was too small to be
perfused in only 1 dog, and our results suggest that the flushing method is preferable to
the mincing method for collecting sperm from the canine cauda epididymis. |
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| Keywords: | canine collection method epididymal sperm frozen semen |
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