| 桑树茎尖嫁接技术研究 |
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| 引用本文： | 马凤桐,郭春慧.桑树茎尖嫁接技术研究[J].蚕业科学,1996,22(1):1-4. |
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| 作者姓名： | 马凤桐 郭春慧 |
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| 作者单位： | 陕西省黄土高原治理研究所 |
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| 摘 要： | 在无菌条件下将桑籽接种至含ＭＳ无机盐与０．２％凝胶剂（Ｇｅｌｒｉｔｅ）的培养基上，于２６℃每日１６ｈ、１０００１ｘ照明下培养；２周后以激素处理茎切段，转培于蛭石培养基，１０～１２ｄ苗茎围度达８ｍｍ时，以长２ｃｍ的切段为砧木，以７０７等桑品种的１～２ｍｍ茎尖作接穗，嫁接成活率达７５％～８０％。增大砧木苗基的围度是取得桑树茎尖嫁接成功的关键。
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| 关 键 词： | 桑树，茎尖嫁接，砧木，接穗 |
A STUDY ON SHOOT APEX GRAFTING OF MULBERRY IN VITRO |
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| Ma Fengtong,Guo Chunhui,Li Minxia, Ma Tiejun,Liu Yurong.A STUDY ON SHOOT APEX GRAFTING OF MULBERRY IN VITRO[J].Acta Sericologica Sinica,1996,22(1):1-4. |
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| Authors: | Ma Fengtong Guo Chunhui Li Minxia Ma Tiejun Liu Yurong |
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| Abstract: | A procedure of shoot-apex grafting in vitro of mulberry is described. Mulberry seeds were inoculated on Murashige and Skoog(MS) medium solidified with 0.2%Gel-rite under aseptic conditions and incubated in a chamber at 26℃,16h daily exporsure to1000lx illumination for two weeks. Sboots were excised with their bases soaked in hor-mone solution and transferred to vermiculite medium, incubated in the chn1ber at 26℃and constant darkness for l0～12 days, When the circumference of the seedling stemsreached about 8mm,they were served as rootstocks for shoot-apex grafting in vitro. Anaverage frequency of 75%～80%of successful shoot-apex grafting of several major com-mercial varieties was achieved by using 2 cm segments from the seedlings as rootstocksand l～2mm shoot apices from adult plants as scions,It is concluded that thickening thecircumference of rootstock stems is the most critical factor for successful shoot-apexgrafting in vilro of mulberry. |
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| Keywords: | Mulberry Shoot apex grafting Rootstock Scion |
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