| ClassI新城疫病毒强毒株融合蛋白的表达时相分析 |
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| 引用本文： | 孙英杰,陈鸿军,仇旭升,詹媛,于洋,宋翠萍,于圣青,丁铲.ClassI新城疫病毒强毒株融合蛋白的表达时相分析[J].中国兽医寄生虫病,2010,18(1):17-22. |
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| 作者姓名： | 孙英杰 陈鸿军 仇旭升 詹媛 于洋 宋翠萍 于圣青 丁铲 |
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| 作者单位： | 中国农业科学院,上海兽医研究所,上海,200241 |
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| 基金项目： | 中央公益性研究院所基础业务专项 |
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| 摘 要： | 根据ClassI新城疫病毒分离株9a5b编码序列，设计特异性引物扩增融合蛋白（F）基因截短片段（199～1500nt），利用原核表达获得重组蛋白，切胶免疫Balb／c小鼠，制备特异性多抗血清，利用制得的血清进行F蛋白表达时相分析。结果表明：NDV9a5b病毒按5M．0．1（multiplicityofinfection，多重感染复数）感染量接种DF1单层细胞，经IFA检测，在感染6h时，F蛋白开始出现，主要分布于细胞浆内；12～16h胞浆申的F蛋白含量逐渐增多，形成包涵体，开始在细胞膜上分布；当病毒感染24h后，F蛋白在细胞膜上形成明显的膜周染色；至感染36h后，出现多个细胞融合的包涵体，细胞已基本崩解。此时，F蛋白也散在分布于包涵体中。这为深入开展F蛋白与该病毒的毒力演化机制研究工作奠定了基础。
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| 关 键 词： | 新城疲病毒 Class I 融合蛋白 动态分布 |
EXPESSION PHASE ANALYSIS OF FUSION PROTEIN OF A VIRULENT STRAIN FROM CLASS I NEWCASTLE DISEASE VIRUS |
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| SUN Ying-jie,CHEN Hong-jun,QIU Xu-sheng,ZHAN Yuan,YU Yang,SONG Cui-ping,YU Sheng-qing,DING Chan.EXPESSION PHASE ANALYSIS OF FUSION PROTEIN OF A VIRULENT STRAIN FROM CLASS I NEWCASTLE DISEASE VIRUS[J].Chinese Journal of Veterinary Parasitology,2010,18(1):17-22. |
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| Authors: | SUN Ying-jie CHEN Hong-jun QIU Xu-sheng ZHAN Yuan YU Yang SONG Cui-ping YU Sheng-qing DING Chan |
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| Institution: | SUN Ying-jie,CHEN Hong-jun,QIU Xu-sheng,ZHAN Yuan,YU Yang,SONG Cui-ping,YU Sheng-qing,DING Chan (Shanghai Veterinary Research Institute,CAAS,Shanghai200241,China) |
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| Abstract: | A truncated fusion gene segment(199nt to 1500nt) was amplified by using a pair of primers designed according to published sequence of Class I Newcastle disease virus(NDV) 9a5b strain.After prokaryotic expression in E.Coli, fusion protein(F protein) was administered into Balb/c mice for preparation of specific polyclonal antibodies,which was used to analyze the expression phase and sublocalization of fusion protein.The DF- 1 monolayer cells were infected by 5 M.O.I ofNDV 9a5b strain and then examined by indi... |
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| Keywords: | Newcastle disease virus （NDV） Class I fusion protein prokaryotic expression phase analysis |
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