DNA fingerprinting,metalaxyl resistance and mating type determination of the<Emphasis Type="Italic">Phytophthora</Emphasis> infestans population in the Republic of Ireland |
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| Authors: | D Griffin E O’Sullivan M A Harmey L J Dowley |
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| Institution: | (1) Teagasc Crops Research Centre, Oakpark, Carlow, Republic of Ireland;(2) Botany Department, UCD, Belfield, Dublin 4, Republic of Ireland |
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| Abstract: | Summary During the period 1995–1999 one hundred and sixty three isolates of P. infestans were collected in Ireland. The majority (150)
were from the 1996 population. Isolates were analysed for mating type and sensitivity to metalaxyl, and subsets were also
genotyped for mitochondrial haplotype (124) and RG-57 fingerprint (114). Four of the isolates tested were of the A2 mating-type. Of four haplotypes known, only two were detected in the Irish population. RG-57 probe analysis determined that
there were 12 genotypes present. However, clustering analysis demonstrated close relationships between many of the strains
found. Metalaxyl resistance was predominantly associated with two closely-related genotypes. All A2 isolates shared the same genotype. Due to the low population diversity, especially of A2, sexual reproduction does not appear to be a factor in disease epidemiology. |
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| Keywords: | RG-57 oospore potato Solanum tuberosum L |
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