| 葡萄籽原花青素及维生素E对氧化应激仔猪生长性能、 血清氧化还原状态和肝脏氧化损伤的影响 |
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| 引用本文： | 赵娇,周招洪,梁小芳,毛湘冰,陈代文,余冰.葡萄籽原花青素及维生素E对氧化应激仔猪生长性能、 血清氧化还原状态和肝脏氧化损伤的影响[J].中国农业科学,2013,46(19):4157-4164. |
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| 作者姓名： | 赵娇 周招洪 梁小芳 毛湘冰 陈代文 余冰 |
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| 基金项目： | 四川省科技支撑计划项目（2012NZ0001） |
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| 摘 要： | 【目的】研究饲粮添加葡萄籽原花青素(grape seed procyanidins, GSPs)和维生素E(vitamin E,VE)对氧化应激仔猪生长性能、血清氧化还原状态及肝脏氧化损伤的影响。【方法】24头28 d的断奶仔猪(L×Y)，随机分为4组，每组6个重复，每个重复1头猪。NC组和Diqaut组饲喂基础饲粮，GSPs+Diquat组和VE+Diquat组分别饲喂在基础饲粮中添加100 mg•kg-1 GSPs和50 mg•kg-1 VE的试验饲粮。在试验第10 d对Diquat组、GSPs+Diquat组和VE+Diquat组试猪腹腔注射Diquat（10 mg•kg-1）, NC组注射生理盐水。试验期为17 d。【结果】结果显示，与NC组相比，注射Diquat导致仔猪生长性能，血清和肝脏抗氧化能力以及肝脏ALT和AST活性显著降低（P＜0.05），血清ALT和AST活性显著升高（P＜0.05）；饲粮添加100 mg•kg-1 GSPs或50 mg•kg-1 VE 均显著改善应激仔猪血清GSH-px活性和抗 能力及肝脏ALT和AST活性(P＜0.05)，降低血清ALT、AST活性和MDA含量(P＜0.05)。与此同时，GSPs还显著增加仔猪血清SOD活性、抗•OH能力及肝脏T-AOC和抗•OH能力(P＜0.05)，降低肝脏MDA含量(P＜0.05)。【结论】饲粮添加100 mg•kg-1 GSPs或50 mg•kg-1 VE均能有效缓解Diquat所致仔猪氧化应激，且在血清和肝脏抗氧化方面GSPs效果较好。
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| 关 键 词： | 葡萄籽原花青素 维生素E 断奶仔猪 氧化应激 肝脏 |
| 收稿时间： | 2013-03-18 |
Effects of GSPs and VE on Growth Performance,Serum Redox Status and Hepatic Oxidative Damage in Piglets Under Oxidative Stress |
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| ZHAO Jiao,ZHOU Zhao-Hong,LIANG Xiao-Fang,MAO Xiang-Bing,CHEN Dai-Wen,YU Bing.Effects of GSPs and VE on Growth Performance,Serum Redox Status and Hepatic Oxidative Damage in Piglets Under Oxidative Stress[J].Scientia Agricultura Sinica,2013,46(19):4157-4164. |
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| Authors: | ZHAO Jiao ZHOU Zhao-Hong LIANG Xiao-Fang MAO Xiang-Bing CHEN Dai-Wen YU Bing |
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| Institution: | Institution of Animal Nutrition, Sichuan Agricultural University/Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Ya’an 625014, Sichuan |
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| Abstract: | 【Objective】 The aim of this study was to investigate the effects of dietary grape seed procyanidins (GSPs) or vitamin E (VE) on growth performance, serum redox status and liver oxidative damage in piglets under oxidative stress. 【Method】 Twenty-four weaned piglets (L×Y) at 28 d of age were allotted into 4 groups with 6 replicates per group and 1 piglet per replicate. Piglets in NC group or Diquat group were fed with basal diet, and those in GSPs+Diquat group or VE+Diquat group were fed with basal diets supplemented with 100 mg•kg-1 GSPs or 50 mg•kg-1 VE, respectively. On d 10, all the piglets in Diquat, GSPs+Diquat and VE+Diquat groups were injected intraperitoneally with Diquat (10 mg•kg-1), and those in NC group were injected with the same amount of sterile saline. The experiment lasted for 17 days. 【Result】 The results showed that Diquat injection caused the reduction of growth performance, anti-oxidant capacities of serum and liver, activities of hepatic ALT and AST(P＜0.05), while increased serum ALT and AST activities (P＜0.05). 100 mg•kg-1 GSPs or 50 mg•kg-1 VE supplementation improved serum GSH-px activity, capacity of anti-superoxide anion as well as hepatic ALT and AST activities (P＜0.05), and decreased ALT and AST activities and MDA content in serum (P＜0.05). Furthermore, GSPs also significantly enhanced serum SOD activity and anti-hydroxyl radical capacity, improved hepatic T-AOC and capacities of anti-hydroxyl radical, and reduced MDA content in liver (P＜0.05). 【Conclusion】Dietary supplementation of 100 mg•kg-1 of GSPs or 50 mg•kg-1 of VE can suppress the oxidative stress caused by Diquat injection in piglets, and GSPs exert a better antioxidant capacity in serum and liver. |
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| Keywords: | grape seed procyanidins vitamin E weaned piglets oxidative stress liver |
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