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| 高原超细型细毛羊经济性状的微卫星标记研究 | |
| 引用本文： | 梁春年,姚军,杨博辉,赵有璋,程胜利,王保全.高原超细型细毛羊经济性状的微卫星标记研究[J].畜牧兽医学报,2005,36(12):1265-1269. |
| 作者姓名： | 梁春年 姚军 杨博辉 赵有璋 程胜利 王保全 |
| 作者单位： | 1. 中国农业科学院兰州畜牧与兽药研究所,兰州,730070 2. 甘肃农业大学,兰州,730050 3. 甘肃皇城绵羊育种试验场,张掖,734031 |
| 基金项目： | 基金项目：甘肃省“十五攻关”项目（GS022-A41-052） |
| 摘 要： | 利用3对微卫星引物对70只中国美利奴高原型超细类群个体进行DNA多态性分析,结果表明：3个微卫星座位具有高度多态性,多态信息含量平均为0.739 1,有效等位基因数4.441 9.3个微卫星标记与经济性状间的相关分析表明,微卫星MCM218的160 bp等位基因和BMC1009的288 bp等位基因与中国美利奴高原型超细群体绵羊羊毛细度有极显著的相关性,相关系数分别为-0.956 1和-0.849 1.微卫星BMS1248的145 bp的等位基因与中国美利奴高原型超细型绵羊周岁体重有显著的相关性.相关系数为0.952 3.这一结论初步说明,微卫星MCM218的160 bp等位基因和BMC1009的288 bp的等位基因与中国美利奴高原型超细群体周岁绵羊羊毛细度存在一定程度的连锁,微卫星BMS1248的145 bp的等位基因与中国美利奴高山型超细型绵羊周岁体重也存在一定的连锁关系,为进一步分析细毛羊主要经济性状的连锁标记以及克隆相关基因打下基础. |
| 关 键 词： | 高原超细型细毛羊 微卫星DNA标记 经济性状 |
| 文章编号： | 0366-6964（2005）12-1265-05 |
| 收稿时间： | 2004-11-10 |
| 修稿时间： | 2004-11-10 |
Study on Economic Traits of Alpine Ultra-fine Wool Sheep Using the Microsatellite DNA Technique |
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| LIANG Chun-nian,YAO Jun,YANG Bo-hui,ZHAO You-zhang,CHENA Sheng-li,WANG Bao-quan.Study on Economic Traits of Alpine Ultra-fine Wool Sheep Using the Microsatellite DNA Technique[J].Acta Veterinaria et Zootechnica Sinica,2005,36(12):1265-1269. | |
| Authors: | LIANG Chun-nian YAO Jun YANG Bo-hui ZHAO You-zhang CHENA Sheng-li WANG Bao-quan |
| Institution: | 1. Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; 2. Gansu Agricultural University, Lanzhou 730050, China;3. Gansu Huangcheng Sheep Breeding Farm, Zhangye 734031, China |
| Abstract: | The study was designed to carry out DNA polymorphism analysis of a total of 70 Alpine utra-fine wool sheep(Chinese Merino) using 3 pairs of microsatellite primers.The results showed that 3 microsatellite Loci of the Alpine ultra-fine wool sheep had high diversity,with the average polymorphism information content of 0.739 1 and the number of effective alleles of 4.441 9.The result of the correlation analysis between genetic diversity of microsatellite DNA and economic traits showed that both 160bp alleles of MCM 218 and 288bp alleles of BMC 1009 were highly correlated with wool fitness(P<0.01),with a correlation coefficient of-0.956 1 and-0.849 1 respectively;145bp alleles of BMS1248 were also highly correlated with yearling weight(P<0.05),with a correlation coefficient of 0.952 3.This indicates that there is a linkage between the genetic diversity of microsatellite DNA and economic traits of Alpine ultra-fine wool sheep.Therefore,this is a fundamental study for further looking for markers of both linkage and cloning,and for early selection of the important economic traits of Alpine ultra-fine wool Chinese merino. |
| Keywords: | Alpine ultra-fine wool sheep microsatellite DNA economic traits |
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