| 酞酸酯污染土壤微生物效应与过氧化氢酶活性的变化特征 |
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| 引用本文： | 高军,陈伯清.酞酸酯污染土壤微生物效应与过氧化氢酶活性的变化特征[J].水土保持学报,2008,22(6). |
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| 作者姓名： | 高军 陈伯清 |
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| 作者单位： | 1. 淮阴工学院,生命科学与化学工程学院,江苏,淮安,223003;江苏省环洪泽湖生态农业重点实验室,江苏,淮安,223300;淮安市农业废弃物与新型基质工程研究中心,江苏,淮安,223003
2. 淮阴工学院,生命科学与化学工程学院,江苏,淮安,223003;淮安市农业废弃物与新型基质工程研究中心,江苏,淮安,223003 |
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| 基金项目： | 江苏省教育厅青蓝工程项目，江苏省淮安市自然科学基金，江苏省环洪泽湖生态农业重点实验室资助项目，淮阴工学院自然科学基金计划 |
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| 摘 要： | 采用室内模拟污染土壤,研究了邻苯二甲酸二(乙基-己基)酯和邻苯二甲酸二丁酯两种酞酸酯类化合物对农田土壤微生物生物量碳、土壤基础呼吸与土壤过氧化氢酶活性及其反应动力学参数的影响。结果表明,DBP土壤浓度在10 mg/kg,DEHP土壤浓度在20 mg/kg以下,与对照相比,微生物生物量碳、土壤基础呼吸以及过氧化氢酶活性没有明显影响。土壤浓度为20 mg/kg的DBP处理对土壤基础呼吸有明显的激活作用。两种化合物在高浓度处理的土壤中,均对微生物生物量碳、土壤基础呼吸以及过氧化氢酶活性表现抑制效应,抑制作用随处理浓度的增加而加强,其中两种化合物土壤浓度为100 mg/kg时,在培养期内三者均没有表现出明显的恢复趋势。对过氧化氢酶Km与Vmax以及Vmax/Km等动力学参数分析表明,DBP与DEHP没有对过氧化氢酶促反应速率以及酶与底物亲和力产生明显影响。
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| 关 键 词： | 酞酸酯 土壤微生物效应 过氧化氢酶 动力学参数 |
Effects of PAEs on Soil Microbial Activity and Catalase Activity |
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| GAO Jun,CHEN Bo-qing.Effects of PAEs on Soil Microbial Activity and Catalase Activity[J].Journal of Soil and Water Conservation,2008,22(6). |
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| Authors: | GAO Jun CHEN Bo-qing |
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| Institution: | GAO Jun1,2,3 CHEN Bo-qing1,3 |
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| Abstract: | The effects of DBP and DEHP on microbial biomass,soil basal respiration,catalase activity and its kinetics parameters(Km and Vmax) in agro-soil contaminated artificially with DBP and DEHP were studied.The results showed that there were little effects on them with no more than 10 mg/kg DBP or 20 mg/kg DEHP in soils.Soil basal respiration was significantly activated with 20 mg/kg DBP in soil.However,Microbial biomass C,basal respiration and catalase activity were all inhibited with other treatments and the greater inhabitations were exhibited with the higher concentrations of compounds in soils.When soil concentrations of compounds were 100 mg/kg,the restoration tendencies of catalase activities were no detected within incubation term.The kinetics parameters of catalase activities further analysis showed that Km,Vmax and Vmax/Km were insignificantly changed compared with the controls,which reflected the compounds,whether DBP or DEHP,had little effect on the kinetics characters of catalase activities in soils. |
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| Keywords: | PAEs soil microbial effects catalase activity kinetics parameters |
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