| 极度濒危植物百山祖冷杉水培繁殖 |
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| 引用本文： | 饶龙兵,吴友贵.极度濒危植物百山祖冷杉水培繁殖[J].农业科学与技术,2009,10(3):43-45,54. |
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| 作者姓名： | 饶龙兵 吴友贵 |
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| 作者单位： | 饶龙兵,RAO Long-bing(中国林业科学研究院亚热带林业研究所,浙江富阳,311400);吴友贵,WU You-gui(百山祖自然保护区,浙江庆元,323800) |
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| 基金项目： | 浙江省科技计划项目，国家自然科学基金 |
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| 摘 要： | 1材料与方法1．1材料试材为百山祖保护区利用日本冷杉作砧木剪接繁殖的木质化枝条，粗0．2-0．5cm，生长健壮，无病虫害，每段枝条8-12cm，留部分叶片，每枝均含顶芽。剪好的枝条放在0．5％KMnO4溶液中，消毒30min，然后用清水冲洗3遍，备用。1．2方法试验因子：培养液，清水和霍格兰营养液；植物激素，10mg／L吲哚乙酸（IAA）和10mg／I。生根粉（ABT）。
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| 关 键 词： | 濒危植物 百山祖冷杉 水培繁殖 病虫害 |
Propagation of Abies beshanzuensis by Water Cultured Medium |
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| RAO Long-bing,WU You-gui.Propagation of Abies beshanzuensis by Water Cultured Medium[J].Agricultural Science ＆ Technology,2009,10(3):43-45,54. |
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| Authors: | RAO Long-bing WU You-gui |
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| Institution: | 1.The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400; 2. Baishanzu Nature Reserve, Qingyuan 323800) |
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| Abstract: | Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly. |
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| Keywords: | Abies beshanzuensis M H Wu Propagation Water cultured Medium Phytohormones Solution |
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