Intra- and intergenomic relationships in interspecific hybrids between Brassica (B. rapa, B. napus) and a wild species B. maurorum as revealed by genomic in situ hybridization (GISH) |
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| Authors: | Xing-Cheng Yao Xian-Hong Ge Ji-Peng Chen and Zai-Yun Li |
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| Institution: | (1) National Key Lab of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070 Wuhan, People’s Republic of China; |
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| Abstract: | Interspecific hybridization plays a crucial role in plant genetics and breeding. The efficiency of interspecific crosses to
a considerable extent depends on the genetic relatedness of genomes from parental species. Interspecific hybrids involving
Brassica maurorum (2n = 16, MM) and two Brassica crop species, viz B. rapa (2n = 20, AA) and B. napus (2n = 38, AACC), were produced and analyzed for their meiotic chromosome pairings in pollen mother cells (PMCs) by using genomic
in situ hybridization (GISH) with the labeled DNA of B. maurorum (MM) as probe. In hybrids B. maurorum × B. rapa (2n = 18, MA), all chromosomes remained unpaired in 28% PMCs, and the maximum of autosyndetic bivalents was two and one among
the chromosomes of A and M genomes, with the average per cell being 0.27 and 0.12, respectively. Up to two allosyndetic bivalents
between A and M genomes appeared, averagely 0.48 per cell. In hybrids B. maurorum × B. napus (2n = 27, MAC), the maximum of autosyndetic bivalents in M genome was two and the average was 0.11, while the maximum of allosyndetic
bivalents between M and A/C genomes was two and the average was 0.78. The 2–7 bivalents formed by A/C-genome chromosomes showed
their high homology. The results were compared and discussed with the chromosome pairings in the hybrids of B. maurorum with B. juncea and B. carinata with respect to the genome relationships and the potential for chromosome recombination. |
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