| 黄芩愈伤组织培养与快速繁殖条件的优化研究 |
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| 引用本文： | 张东向,李富雄.黄芩愈伤组织培养与快速繁殖条件的优化研究[J].安徽农业科学,2008,36(29). |
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| 作者姓名： | 张东向 李富雄 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006;齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省骨干教师项目
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| 摘 要： | 目的]培养黄芩优良品种并进行克隆化快速繁育。方法]用黄芩(Scutellaria baicalensisGeorgi)无菌根作为外植体进行愈伤组织的诱导、分化,黄芩试管苗的继代扩大繁殖、生根等一系列优化试验。结果]MS+2.0 mg/L 6-BA+0.2 mg/L NAA培养基进行愈伤组织的分化培养效果最好;MS+2.0 mg/L 6-BA+0.2 mg/L NAA进行试管苗的复壮效果较好;最有效的生根培养基为1/2 MS+0.3mg/L NAA,生根率可达96.7%。结论]采用单节培养的方法,可以大幅度提高黄芩繁殖速度。
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| 关 键 词： | 黄芩 愈伤组织 组织培养 快速繁殖 |
Callus Culturing and Optimization of Rapid-propagation Technology of Scutellaria baicalensis Georgi |
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| Abstract: | Objective] The research aimed to culture the fine varieties of Scutellaria baicalensis Georgi and carry out cloning rapid-propagation.Method] Research by sterile root explants of S.baicalensis Georgi including callus inducing,differentiation,plantlet propagation and rooting were designed.Result] The results showed that MS+2.0 mg/L 6-BA+0.2 mg/L NAA was the best medium for callus differentiation and plantlet rejuvenation.The best medium was 1/2 MS+0.3 mg/L NAA for rooting,and the rooting rate reached 96.7%.Conclusion] The propagation rate could be improved using single node for S.baicalensis Georgi. |
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| Keywords: | Scutellaria baicalensis Georgi Callus Tissue culture Rapid-propagation |
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