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枸杞叶片DNA不同提取方法比较
引用本文:思彬彬,尚洁,马艳辉.枸杞叶片DNA不同提取方法比较[J].安徽农业科学,2009,37(15):6872-6872.
作者姓名:思彬彬  尚洁  马艳辉
作者单位:北方民族大学生命科学与工程学院,宁夏,银川,750021;北方民族大学生命科学与工程学院,宁夏,银川,750021;北方民族大学生命科学与工程学院,宁夏,银川,750021
摘    要:目的]为研究枸杞的遗传多样性、种质鉴定和指纹图谱的构建提供基础保证。方法]以枸杞成熟叶片为试材,分别采用常规CTAB法、高盐CrAB法等11种方法从枸杞中提取基因组DNA,比较不同方法的提取效果。结果]11种方法中,除5XCTAB法试验结果不理想外,其余10种均能有效地从枸杞叶片中获得DNA,所提DNA的质量和产量均无明显差异。结论]11种方法提取的枸杞基因组DNA,能够满足RAPD分析的需要。

关 键 词:枸杞  成熟叶片  DNA提取

Comparison of Different Extraction Methods of Genomic DNA from the Leaves of Lycium barbarum L.
Institution:SI Bin-bin et al (College of Life Science and Enghaeering,Northern University for Natio'nalities,Yinchuan,Ningxia 750321)
Abstract:Objective] The research aimed to provide basic guarantee for studying the genetic diversity the germplase identification and construction of fingerprint of Lycium barbarum L.Method] With mature leaves of Lycium barbarum L.as tested material,the genomic DNA was extracted from Lycium barbarum L.using eleven different methods,such as CTAB method,high-salt CTAB method and so on.The extraction effects of different methods were compared.Result] High-quality DNA could be obtained with ten of all methods,except 5×CTAB method.Quality and outtput of DNA had no significant differences.Conclusion]The genomic DNA could be extracted from the mature leaves of Lycium barbarum L.by 11 kinds of methods.These methods all could be used in RAPD reaction.
Keywords:Lycium barbarum L    Mature leaves  DNA extraction
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