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落叶松八齿小蠹伴生真菌3个菌株的鉴定及生物学生理学特性
引用本文:周秀华,宋瑞清,曹翠,崔磊,梁晓东,潘建中,朱元金,胡振宇.落叶松八齿小蠹伴生真菌3个菌株的鉴定及生物学生理学特性[J].林业科学,2011,47(5).
作者姓名:周秀华  宋瑞清  曹翠  崔磊  梁晓东  潘建中  朱元金  胡振宇
作者单位:1. 东北林业大学林学院,哈尔滨150040;长春大学生物科学技术学院,长春130012
2. 东北林业大学林学院,哈尔滨150040;黑龙江省林业科学院,哈尔滨150081
3. 东北林业大学林学院,哈尔滨,150040
4. 哈尔滨工业大学食品科学与工程学院,哈尔滨,150010
5. 黑龙江省佳木斯市孟家岗林场,桦南,154411
摘    要:从落叶松八齿小蠹各虫态体内外、坑道及木材蓝变部位获得3株与落叶松八齿小蠹伴生的真菌,该3株菌可导致木材变色,与蠢害的发生存在密切关系.通过菌体的培养特性和形态观察、放线菌酮耐性及rDNA ITS序列测定,将3菌株确认为Ceratocystis fujiensis,为国内新记录种.3个菌株生长的最佳环境条件:温度为25℃,光照为全黑暗条件,pH值为5.0;最佳营养条件:培养基为PDA+落叶松韧皮培养基,碳源为蔗糖,氮源为酵母粉.3个菌株均具有较强的高温和低温耐受性,能够适应北方较大的温差,树体内的黑暗和酸性条件也比较适合菌株的生长.3个菌株对寄主落叶松具有明显的营养选择性,可利用的碳源种类较多,有机氮源利于菌株生长.

关 键 词:落叶松八齿小蠢  伴生真菌  生物学特性  生理学特性

Identification and Biological and Physiological Characteristics of 3 Fungus Strains Associated with Ips subelongatus
Zhou Xiuhua,Song Ruiqing,Cao Cui,Cui Lei,Liang Xiaodong,Pan Jianzhong,Zhu Yuanjin,Hu Zhenyu.Identification and Biological and Physiological Characteristics of 3 Fungus Strains Associated with Ips subelongatus[J].Scientia Silvae Sinicae,2011,47(5).
Authors:Zhou Xiuhua  Song Ruiqing  Cao Cui  Cui Lei  Liang Xiaodong  Pan Jianzhong  Zhu Yuanjin  Hu Zhenyu
Institution:Zhou Xiuhua1,2 Song Ruiqing1,3 Cao Cui1 Cui Lei4 Liang Xiaodong5 Pan Jianzhong5 Zhu Yuanjin5 Hu Zhenyu5(1.Forestry College,Northeast Forestry University Harbin 150040,2.Bio-Scientific and Technical College,Changchun University Changchun 130012,3.Heilongjiang Forestry Academy of Science Harbin 150081,4.School of Food Science and Engineering,Harbin Insititute of Technology Harbin 150010,5.Mengjiagang Forest Farm of Jiamusi,Heilongjiang Province Huanan 154411)
Abstract:Three fungal strains associated with Ips subelongatus were isolated from the blue-stain tissue,inside and surface of the body of Ips subelongatus.The fungal stains can cause wood sapstains which associate with the bark beetle attacks.According to culture and morphology characteristic,cycloheximide tolerance and rDNA ITS molecular sequence,the three strains were identified as Ceratocystis fujiensis M.J.Wingf.which is a new record species in China.The optimum conditions for strains growth were as follows:temp...
Keywords:Ceratocystis  fujiensis
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