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| TCS基因转化泡桐及抗病能力 | |
| 引用本文： | 刘静,黄艳艳,翁曼丽,罗磊,张虹,王长宪,牛庆霖,冯殿齐.TCS基因转化泡桐及抗病能力[J].林业科学,2011,47(5). |
| 作者姓名： | 刘静 黄艳艳 翁曼丽 罗磊 张虹 王长宪 牛庆霖 冯殿齐 |
| 作者单位： | 1. 泰山林业科学研究院,泰安,271000 2. 中国科学院遗传与发育生物学研究所,北京,100101 3. 山东农业大学,泰安,271018 |
| 摘 要： | 泡桐(Paulownia)是经济价值较高的优良绿化造林树种.由于丛枝病的危害,每年我国林业的直接经济损失就高达数千万元,并且严重影响农民种植泡桐的积极性(杨俊秀等,2007). |
| 关 键 词： | 泡桐 TCS基因 农杆菌介导 稳定性 病毒抗性 |
Transformation of TCS Gene to Paulownia elongata and Its Virus Resistance |
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| Liu Jing,Huang Yanyan,Weng Manli,Luo Lei,Zhang Hong,Wang Changxian,Niu Qinglin,Feng Dianqi.Transformation of TCS Gene to Paulownia elongata and Its Virus Resistance[J].Scientia Silvae Sinicae,2011,47(5). | |
| Authors: | Liu Jing Huang Yanyan Weng Manli Luo Lei Zhang Hong Wang Changxian Niu Qinglin Feng Dianqi |
| Institution: | Liu Jing1 Huang Yanyan1 Weng Manli2 Luo Lei1 Zhang Hong1 Wang Changxian1 Niu Qinglin3 Feng Dianqi1(1.Taishan Institute of Forestry Science Tai'an 271000,2.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences Beijing 100101,3.Shandong Agricultural University Tai'an 271018) |
| Abstract: | In this study,the trichosanthin(TCS) gene,which was cloned from Trichosanthes kirilowii,was transformed into Paulownia elongata through agrobacterium-mediated method.The conversion rate was 29.2% with 86 transformed strains obtained.The target gene had approximately 50% positive expression rate in plantlets through 11 times subculture and PCR detection for 4 times.And the PCR detection of the plants in field showed that the positive rate was between 40% and 50%.No positive expression of the labeled product ... |
| Keywords: | Paulownia elongata TCS gene agrobacterium-mediated stability virus resistance |
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