| 禽呼肠孤病毒C-98、T-98分离株S4基因的克隆与序列分析 |
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| 引用本文： | 党娟,霍金山,何建文,关平原.禽呼肠孤病毒C-98、T-98分离株S4基因的克隆与序列分析[J].内蒙古农业大学学报(自然科学版),2012,33(2):1-6. |
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| 作者姓名： | 党娟 霍金山 何建文 关平原 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院,呼和浩特,010018
2. 内蒙古兴安盟动物疫病预防控制中心,乌兰浩特,137400 |
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| 基金项目： | 内蒙古自然科学基金资助项目 |
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| 摘 要： | 参考GenBank中禽呼肠孤病毒176株(ARV 176)S4基因序列设计两对引物,分别提取禽呼肠孤病毒T-98和C -98分离株病毒总RNA,应用RT - PCR技术扩增病毒的S4基因,将纯化的目的DNA与pEASY - T1载体连接、转化后测序.应用计算机软件将所测定序列与参考毒株序列进行比较,结果显示,S4基因核苷酸序列长为1 192bp,均含1个完整的开放性阅读框(24-1 127).ARV T - 98和ARV C - 98分离株S4基因的核苷酸同源性很高,为99.9％；与参考毒株ARV 1733、ARV S1133、ARV 750505、ARV 601SI、ARV 919、ARV T6、ARV OS161和DRV YJL S4基因的核苷酸同源性均较高,在99.4％ -99.9％之间；与ARV 601G、ARV 1017 -1、ARV 916、ARV 918和DRV S14S4基因的核苷酸同源性在80.1％ -83.6％之间；与NBV、BRV和MRV 3的S4基因核苷酸同源性在2.4％-53.1％之间.进化树分析显示:11株禽呼肠孤病毒分离株和ARVT - 98、C -98分离株的S4基因可分成3个不同的系谱.
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| 关 键 词： | 禽呼肠孤病毒 S4基因 克隆 列分析 |
CLONING AND SEQUENCE ANALYSIS OF S4 GENE OF AVIAN REOVIRUS C -98、T -98 ISOLATES |
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| DANG Juan
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HUO Jin-shan
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HE Jian-wen
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CUAN Ping-yuan.CLONING AND SEQUENCE ANALYSIS OF S4 GENE OF AVIAN REOVIRUS C -98、T -98 ISOLATES[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(2):1-6. |
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| Authors: | DANG Juan HUO Jin-shan HE Jian-wen CUAN Ping-yuan |
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| Institution: | 1*(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China; 2.Center of Prevent ang Control of Animal Disease of Xinan Meng of Inner Mongolia,Wulanhot 137400,China) |
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| Abstract: | According to the S4 gene sequence of Avian reovirus strain 176(ARV 176) published in GenBank,two pairs of primers were synthesized.The total RNA of ARV isolate T-98 and C-98 isolated in Tianjin and inner Mongolia were extracted.The S4 gene of virus was amplificated by RT-PCR and then cloned into pESAY-T1 plasmid and then sequenced.The sequences were compared with reference strains gene sequences by computer software.The results showed that S4 genes were 1192bp and contained one ORF(24~1127).Comparision of nucleotide sequence showed that high homology was 99.9% between ARV T-98 and ARV C-98.The homology of S4 gene was about 99.4%~99.9% compared with reference strains ARV 1733、ARV S1133、ARV 750505、ARV 601SI、ARV 919、ARV T6、ARV OS161 、DRV YJL and 80.1%~83.6% compared with reference strains ARV 601G、ARV 1017-1、ARV 916、ARV 918、DRV S14 and 2.4%~53.1% compared with reference strains NBV、BRV、MRV 3.phylogenetic analysis of S4 gene of 11 ARV strains and ARV T-98、C-98 showed that these genes have separated into three lineages. |
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| Keywords: | Avian reovirus S4 gene Clone Sequence Analysis |
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