| 马铃薯黑痣病菌毒素诱导马铃薯幼苗丙二醛含量、细胞膜透性及PAL活性的变化 |
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| 引用本文： | 张笑宇,于肖夏,高翔,于卓,琚亮亮,薛玉凤.马铃薯黑痣病菌毒素诱导马铃薯幼苗丙二醛含量、细胞膜透性及PAL活性的变化[J].内蒙古农业大学学报(自然科学版),2012,33(2):16-20. |
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| 作者姓名： | 张笑宇 于肖夏 高翔 于卓 琚亮亮 薛玉凤 |
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| 作者单位： | 1. 内蒙古农业大学农学院,呼和浩特,010019
2. 中国农业大学农业部作物遗传改良重点实验室,北京,100193 |
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| 基金项目： | "十二五"国家科技支撑计划课题，内蒙古科技创新引导奖励资金项目，呼和浩特市农业综合开发科技创新项目，内蒙古自然科学基金 |
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| 摘 要： | 本试验选取对黑痣病菌抗性不同的马铃薯材料,研究黑痣病菌毒素对马铃薯幼苗体内丙二醛( MDA)含量、细胞膜透性及苯丙氨酸解氨酶(PAL)活性的变化及其与抗病性的关系.结果显示,经毒素处理后,马铃薯幼苗体内MDA含量大幅增加,96 h以前感病品种大西洋的累积量大于抗病品种底西芮的,96h以后抗病品种的累积量大于感病品种的；马铃薯幼苗细胞膜透性增加,感病品种增加的较早且幅度较大；黑痣病菌毒素可诱导马铃薯幼苗体内PAL活性升高,抗病品种升高较早且幅度较大,抗病品种和感病品种的PAL活性分别在12 h和36 h达到最大,增幅分别为48.8％和38.7％,24h以后,二者的增幅没有差异.
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| 关 键 词： | 马铃薯 黑痣病菌 毒素 丙二醛 细胞膜透性 苯丙氨酸解氨酶 |
THE CHANGES ON MDA CONTENT, CELL MEMBRANE PERMEABILITY AND PAL ACTIVITY AFTER INOCULATING WITH TOXIN OF Rhizoctonia soLani IN POTATO |
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| ZHANG Xiao-yu
,
YU Xiao-xia
,
GAO Xiang
,
YU Zhuo
,
JU Liang-liang
,
XUE Yu-feng.THE CHANGES ON MDA CONTENT, CELL MEMBRANE PERMEABILITY AND PAL ACTIVITY AFTER INOCULATING WITH TOXIN OF Rhizoctonia soLani IN POTATO[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(2):16-20. |
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| Authors: | ZHANG Xiao-yu YU Xiao-xia GAO Xiang YU Zhuo JU Liang-liang XUE Yu-feng |
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| Institution: | 1(1.College of Agronomy,Inner Mongolia Agricultural University,Huhhot 010019,China; 2.Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture, China Agricultural University,Beijing 100193,China) |
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| Abstract: | The relationship between the malondialdehyde(MDA) content,cell membrane permeability,phenylalanine ammonia lyase(PAL) activity and the resistance to Rhizoctonia solani in potato inoculated with it’s toxin was studied with potato seedling of having different resistance.The results showed MDA content,cell membrane permeability and PAL activity were all increased after inoculating.0~96h,MDA content of susceptible cultivar Atlantic was greater than the resistance cultivar Desiree’s.96~168h,MDA content of the resistance cultivar was greater than the susceptible cultivar.The cell membrane permeability of susceptible varieties increased earlier and more.PAL activity of resistant varieties increased earlier and more.Both reached maximum at 12h and 36h,rate of increase was 48.8% and 38.7%.24h After inoculating,PAL activity has not significant different between resistant and susceptible varietie. |
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| Keywords: | Potato Rhizoctonia solani toxin MDA cell membrane permeability PAL |
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