| 葱兰的离体培养及再生体系研究 |
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| 引用本文： | 邓祖丽颖,袁秀云.葱兰的离体培养及再生体系研究[J].中国农学通报,2013,29(13):140-144. |
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| 作者姓名： | 邓祖丽颖 袁秀云 |
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| 作者单位： | 1. 郑州幼儿师范高等专科学校,郑州,450000
2. 郑州师范学院生物技术研究所,郑州,450044 |
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| 基金项目： | 郑州市科技攻关项目“珍稀植物引种与快速繁育”(074SGYS33205-2)。 |
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| 摘 要： | 为了快速获得健康的葱兰幼苗，通过优化离体培养条件，建立葱兰高效的离体再生体系。利用葱兰叶片、鳞叶和顶芽作为外植体进行了愈伤组织和植株再生体系研究。结果表明，顶芽为最适外植体；丛生芽诱导和增殖的最适培养基为MS+TDZ 0.5 mg/L+NAA 0.1 mg/L，其次是MS+6-BA 0.5 mg/L+NAA 0.1 mg/L；生根培养基为1/2MS+IBA 0.5 mg/L，生根率为100%，为葱兰的工厂化生产提供技术参考。
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| 关 键 词： | 葱兰 离体培养 再生体系 |
| 收稿时间： | 2012/10/18 0:00:00 |
| 修稿时间： | 2012/10/31 0:00:00 |
Study on Tissue Culture in Vitro and Regeneration System of Zephyranthes candida |
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| Dengzu Liying
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Yuan Xiuyun.Study on Tissue Culture in Vitro and Regeneration System of Zephyranthes candida[J].Chinese Agricultural Science Bulletin,2013,29(13):140-144. |
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| Authors: | Dengzu Liying Yuan Xiuyun |
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| Abstract: | The study aimed to establishment of regeneration system and getting healthy plantlet through optimizing in vitro culture condition. The regeneration system of Zephyranthes candida was established in this paper by using leaves, scale leaves and terminal bud as explants in vitro culture. The results showed that terminal bud was optimum explants for induction. The optimum medium for induction and proliferation of rosette buds was MS+ TDZ 0.5 mg/L+ NAA 0.1 mg/L, followed by MS+ 6-BA 0.5 mg/L + NAA 0.1 mg/L. The rooting medium was 1/2MS+ IBA 0.5 mg/L, the rooting rate reached 100%. The system proved a technological foundation for industrialized production of Zephyranthes candida. |
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| Keywords: | Zephyranthes candida in vitro culture regeneration system |
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