| 11株侧柏内生真菌的农药活性及分子生物学鉴定 |
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| 引用本文： | 王静,易晓华,魏艳,郝双红.11株侧柏内生真菌的农药活性及分子生物学鉴定[J].中国农学通报,2013,29(15):183-187. |
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| 作者姓名： | 王静 易晓华 魏艳 郝双红 |
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| 作者单位： | 1. 青岛农业大学化学与药学院/山东省农业仿生应用工程技术研究中心,山东青岛,266109
2. 青岛农业大学生命科学学院,山东青岛,266109 |
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| 基金项目： | 山东省自然科学基金(联合专项)“具除草活性的侧柏内生真菌J46的生物学特性及其活性成分的分离鉴定”(ZR2011CL007)。 |
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| 摘 要： | 旨在考察11株侧柏内生真菌的抑菌及除草活性，并确定具有较好活性的内生真菌的分类学地位。采用生长速率法测定抑菌活性，采用种子萌发法测定除草活性，菌种鉴定采用ITS序列系统发育分析法。结果表明，在11株侧柏内生真菌中，Y52、J94、Y127、J77、J46等5株内生真菌对苹果腐烂病菌的抑制率高于70%，J94、Y52、J77、J46等4株内生真菌对番茄灰霉病菌抑制率高于70%。内生真菌对反枝苋种子幼根的生长表现出较强抑制作用，抑制率在70%以上的有8株。实验筛选出的8株具有较好活性的侧柏内生真菌均为链格孢属链格孢菌(Alternaria alternaria)，值得进一步研究。
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| 关 键 词： | 侧柏 内生真菌 抑菌活性 除草活性 分子鉴定 |
| 收稿时间： | 2012/11/12 0:00:00 |
| 修稿时间： | 2012/12/24 0:00:00 |
Pesticide Activity and Molecular Biology Identification of 11 Strains of Endophytic Fungi from Oriental Arborvitae |
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| Wang Jing
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Yi Xiaohua
,
Wei Yan
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Hao Shuanghong.Pesticide Activity and Molecular Biology Identification of 11 Strains of Endophytic Fungi from Oriental Arborvitae[J].Chinese Agricultural Science Bulletin,2013,29(15):183-187. |
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| Authors: | Wang Jing Yi Xiaohua Wei Yan Hao Shuanghong |
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| Abstract: | The antifungal and herbicidal activity of 11 strains of endophytic fungi ( J46, J77, J80, J85, J86, J94, Y51, Y52, Y57, Y125, Y127 ) from Oriental Arborvitae against the mycelium growth of 12 kinds of plant pathogenic fungi and seed germination of Amranthus retroflexus were tested respectively. The results showed that the inhibition rate of 10 strains of endophytic fungi were more than 50% at least to one pathogenic fungus. There were 5 strains of endophytic fungi ( Y52, J94, Y127, J77 and J46) whose inhibition rate against Valsa mali were more than 70%, and 4 strains of endophytic fungi (J94, Y52, J77 and J46) whose inhibition rate against Botrytis cinerea were more than 70%. The endophytic fungi displayed strong inhibition to the radicle of A. retroflexus, in which inhibition rate of 8 strains were more than 70%. ITS sequence was determined to classify the 8 strains of endophytic fungi with good antifungal or herbicidal activity, and it showed that they were all Alternaria alternaria. |
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| Keywords: | Platycladus orientalis (L ) Franco endophytic fungus antifungal activity herbicidal activity molecular identification |
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