Functional characterization of Farfantepenaeus californiensis, Litopenaeus vannamei and L. stylirostris haemocyte separated using density gradient centrifugation |
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| Authors: | Francisco Vargas-Albores Teresa Gollas-Galván & Jorge Hernández-López |
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| Institution: | CIAD, Marine Biotechnology, Hermosillo, Son, México; CIBNOR, La Paz, BCS, México |
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| Abstract: | Haemocytes from penaeid shrimp (Farfantepenaeus californiensis, Litopenaeus vannamei and L. stylirostris) were separated using a discontinuous Percoll gradient. Shrimp haemocytes were spontaneously adhered to glass, allowing slide preparations for staining and microscopic differential counting. Like other crustaceans, shrimp has three main populations differing in presence and size of cytoplasmic granules and each population seems to be biochemical or functionally compromised. Prophenoloxidase (proPO )activity was mainly located in large granules haemocytes (75%) while the small granules cells participate with 25%, but seem to be responsible for encapsulation. Haemocyte discrimination ability was tested using Sephadex? (Seph?), DEAE‐Seph? and CM‐Seph?. Only DEAE‐Seph? was encapsulated by shrimp haemocytes and provoked the release of proPO activating system, indicating the role of particle charge in the activation of shrimp immune response. |
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| Keywords: | immune cells haemocyte separation adherence prophenoloxidase Percoll gradient abiotic encapsulation |
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