| 鸡、鹌鹑及其杂交禽胚胎肌肉发育过程中MSTN和p21基因的表达规律 |
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| 引用本文： | 梁耀伟,赵宗胜,陈丹盈,赵松华,张慧,班谦.鸡、鹌鹑及其杂交禽胚胎肌肉发育过程中MSTN和p21基因的表达规律[J].畜牧兽医学报,2012,43(6):997-1002. |
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| 作者姓名： | 梁耀伟 赵宗胜 陈丹盈 赵松华 张慧 班谦 |
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| 作者单位： | 石河子大学动物科技学院,石河子,832003 |
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| 摘 要： | 旨在研究MSTN和p21基因在鸡、鹌鹑和杂交禽胚胎肌肉发育过程中的表达规律,比较这2个基因在杂交禽与亲本鸡和鹌鹑中的差异表达,探讨MSTN和p21基因与肌肉发育的关系。通过人工受精获得鸡(♂)与鹌鹑(♀)杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批入孵,采集第7～17天活胚胸肌组织,应用实时荧光定量PCR技术检测MSTN和p21基因在3个物种中每一天mRNA的相对表达量。MSTN和p21基因在胚胎肌肉发育的第7～17天均有表达,这2个基因mRNA的表达在鸡胚中第9天到达第1次峰值,在鹌鹑中第7天到达第1次峰值,后都随胚胎的发育表达水平上升,并维持相对稳定的高水平表达。杂交禽的表达规律与鸡一致,也在第9天达到峰值,而p21mRNA表达极显著高于鸡和鹌鹑(P<0.01)。MSTN mRNA表达规律与成肌细胞退出细胞周期的时间规律一致;在胚胎肌肉发育过程中,MSTN基因特异性上调p21的表达。
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| 关 键 词： | 鸡 鹌鹑 杂交禽 肌肉发育 MSTN基因 p21基因 实时荧光定量PCR |
The Expresstional Profiles of MSTN and p21 during Embryonic Myogenesis in Chicken,Quail and Hybrids of Chicken-quail |
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| LIANG Yao-wei
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ZHAO Zong-sheng
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CHEN Dan-ying
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ZHAO Song-hua
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ZHANG Hui
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BAN Qian.The Expresstional Profiles of MSTN and p21 during Embryonic Myogenesis in Chicken,Quail and Hybrids of Chicken-quail[J].Acta Veterinaria et Zootechnica Sinica,2012,43(6):997-1002. |
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| Authors: | LIANG Yao-wei ZHAO Zong-sheng CHEN Dan-ying ZHAO Song-hua ZHANG Hui BAN Qian |
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| Institution: | (College of Animal Science and Technology,Shihezi Unversity,Shihezi 832003,China) |
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| Abstract: | The purpose of this study was to analyze MSTN and p21 mRNA expression profiles and differences among chicken,quail and hybrids of chicken-quail during embryonic myogenesis,and evaluate the potential relationships between their relative expression and myogenesis.The cross-bred eggs of quail(♀) were acquired by artificial insemination with chicken(♂)-semen,the eggs of chicken,quail and hybrid were hatched in one batch according to the standard condition of chicken,the breast muscle tissues was collected from day 7 to 17.The expression of MSTN and p21 mRNA in different species every day was revealed by real-time quantitative PCR.The expression of MSTN and p21 mRNA was detected from day 7 to 17.Peak of MSTN and p21 expression was noticed at day 9 in chicken,and day 7 in quail,with the development of muscle,the expression level was rising,which was almost maintained at this level until day 17.And the peak expression was also noticed at day 9 in hybrid which was in accordance with chicken,however,the expression of p21 mRNA in hybrid was significantly higher than that in chicken and quail(P<0.01).The expression profile of MSTN was in accordance with the profile of the withdrawal of myoblasts from the cell cycle,and MSTN could up-regulated the expression of p21 specifically during embryonic myogenesis. |
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| Keywords: | chicken quail hybrid myogenesis MSTN gene p21 gene real-time quantitative PCR |
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