| 鹿特异性复合麻醉剂对大鼠不同脑区ATP酶活性的影响 |
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| 引用本文： | 尹柏双,;王秋竹,;付连军,;呼显生,;石星星,;姜胜,;高利,;王洪斌.鹿特异性复合麻醉剂对大鼠不同脑区ATP酶活性的影响[J].兽医大学学报,2014(11):1842-1845. |
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| 作者姓名： | 尹柏双 ;王秋竹 ;付连军 ;呼显生 ;石星星 ;姜胜 ;高利 ;王洪斌 |
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| 作者单位： | [1]吉林农业科技学院动物医学学院,吉林吉林市1321101; [2]东北农业大学动物医学学院,黑龙江哈尔滨150030 |
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| 基金项目： | 国家自然科学基金资助项目（31302150,31372491）; 长白山动植物资源保护与利用省级重点实验室基金项目（吉农院合字2012701,2013S029）; 吉林农业科技学院博士启动基金项目（吉农院合字2012 307）; 吉林农业科技学院种子基金项目（吉农院合字2013905） |
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| 摘 要： | 为研究鹿特异性复合麻醉剂麻醉与大鼠各脑区突触体ATP酶活性的关系,探讨其麻醉机理。将20只SD大鼠分为对照组和麻醉组,对照组大鼠腹腔注射30mL/kg生理盐水,试验组腹腔注射30mg/kg鹿特异性复合麻醉剂,采集大鼠各脑区,利用比色法测定脑区内Na＋-K＋-ATP、Ca2＋-AT P和Mg2＋-ATP酶活性。结果显示,药物作用后大鼠大脑皮层和脑干Na＋、K＋-ATP酶活性与对照组比较降低显著（P〈0.05或P〈0.01）,小脑、脑干和海马Ca2＋-ATP酶活性与对照组比较显著降低（P〈0.05或P〈0.01）,大脑Mg2＋-AT P酶活性低于对照组（P〈0.05或P〈0.01）。结果表明,麻醉引起大鼠大脑皮层、脑干中Na＋-K＋-ATP酶活性降低,大脑皮层中Mg2＋-ATP酶活性低,小脑、脑干和海马脑区中Ca2＋-ATP酶活性降低可能是鹿特异性复合麻醉剂麻醉作用的机理之一。
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| 关 键 词： | 特异性复合麻醉剂 大鼠 脑区 ATP酶 |
Effects of the specificity anesthetic for deer on ATPase activity in different encephalic region of rats |
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| Institution: | YIN Bai-shuang , WANG Qiu-zhu , FU Lian-jun , HU Xian-sheng , SHI Xing-xing , JIANG Sheng , GAO Li, WANG Hong-bin (1. Department of Animal Medicine, Jilin Agricultural Science and Technology, Jilin City, Jilin 132101, China; 2. College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China) |
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| Abstract: | This experiment was aimed to determine the influence of specificity anesthetic for deer on ATPase activities in different brain regions of rats in order to clarify its general anesthetic mechanism. 20 SD rats were randomly divided into control group, anesthesia group （ip. 30 mg/ kg）. Samples of cerebral cortex, hippocampus, cerebellum, thalamus and brain stem in different period under specificity anesthetic for deer anesthesia were separated by differential centrifugation. The ATPase activities of synaptosome in above brain regions were determined by using col- orimetry methods. The results showed that, the Na＋-K＋-ATPase activities of synaptosomal membranes in cerebral cortex and brain stem were decreased obviously compared with that of the control group （P,0.05 or P〈0.01）. The Ca2＋-ATPase activities of synaptosome in hippocampus, cerebellum and brain stem were decreased （P〈0.05 or P〈0.01）. The Mg^2＋-ATPase activities of synaptosome in cerebral cortex was decreased （P〈0.05 or P〈0.01）. The results indicated that Na＋-K＋-ATPase,Ca^2＋-Mg^2＋-ATPase changed were in above brain regions might be the main mechanism of general anesthetic action by specificity anesthetic for deer. |
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| Keywords: | specificity anesthetic for deer encephalic region rats ATPase |
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