| 粳稻中花11 T-DNA插入突变体的分离和鉴定 |
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| 引用本文： | 郝铭,阎双勇,谭震波,付崇允,夏九成,马炳田,李仕贵.粳稻中花11 T-DNA插入突变体的分离和鉴定[J].西南农业学报,2006,19(5):777-781. |
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| 作者姓名： | 郝铭 阎双勇 谭震波 付崇允 夏九成 马炳田 李仕贵 |
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| 作者单位： | 1. 四川农业大学水稻研究所,四川,温江,611130;作物基因资源与遗传改良教育部重点实验室,四川,雅安,625014
2. 大北农农业科技研究院,北京,100085 |
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| 摘 要： | 通过农杆菌(Agrobacterium tumefaciens)介导法,对中花11成熟胚愈伤组织进行T-DNA转化,构建了含1489个独立的再生转化株系的突变体库,不仅为水稻功能基因组提供了一个有效的研究平台,还创造了一些具有优良农艺性状的突变材料,为水稻育种提供新的基因资源。插入第1代单本移栽且单本收获,第2代播种1066个家系,在水稻各个生育阶段从中独立筛选得到各类外形突变体66个。经过第三代重复筛选获得包括茎、叶、花和穗变异等各类突变52份。结果表明,外观性状的突变频率为3.4%。
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| 关 键 词： | 水稻 T-DNA插入突变 农杆菌 形态性状 |
| 文章编号： | 1001-4829（2006）05-0777-05 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2005-11-15 |
Identification and segregation of character of mutants induced from Zhonghua 11(Oryza sativa L.subsp.japonica) by T-DNA insertion |
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| HAO Ming, YAN Shuang-yong,FU Cong-yun, XIA Jiu-cheng, MA Bin-tian, LI Shi-gui.Identification and segregation of character of mutants induced from Zhonghua 11(Oryza sativa L.subsp.japonica) by T-DNA insertion[J].Southwest China Journal of Agricultural Sciences,2006,19(5):777-781. |
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| Authors: | HAO Ming YAN Shuang-yong FU Cong-yun XIA Jiu-cheng MA Bin-tian LI Shi-gui |
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| Abstract: | T-DNA can be inserted with high efficiency into the genome of the rice variety Zhonghua11(Oryza sativa L.),which was transformed using the Agrobacterium-mediated co-cultivation method,resulting in 1489 independent transgenetic rice plants that carry T-DNA fragments.It not only provids a valid research platformfor the function genomics in rice,but also creates some mutants with the good agriculture characters,and provide some new gene resources for rice breeding.T_1 generation was transplanted and harvested by each single plant.1066 of T_2 generation were planted.66 phenotypic mutants were scanned in term of their performance at every growing stage.52 mutants including stalk,leaf,bloomand panicle variations were identified and selected In T_3 generation repeatedly.The results indicated that the mutation frequency of morphological character was 3.4 %. |
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| Keywords: | rice T-DNA insertional mutagenesis Agrobacterium tumefaciens morphological characteristics |
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