| 玛丽鱼染色体核型及Ag-NORs研究 |
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| 引用本文： | 王琳超,鲍海港,李俊英,吴常信.玛丽鱼染色体核型及Ag-NORs研究[J].大连海洋大学学报,2016(2):151-155. |
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| 作者姓名： | 王琳超 鲍海港 李俊英 吴常信 |
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| 作者单位： | 中国农业大学 动物科技学院 动物遗传资源与分子育种实验室,北京,100193 |
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| 基金项目： | 中国农业大学科技基金资助项目(88057007) |
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| 摘 要： | 为获得玛丽鱼Poecilia latipinna细胞遗传学数据,以玛丽鱼的鳃丝和鳍条为试验材料,采用秋水仙素浸泡、低渗处理和热滴片法制备染色体标本,分析其染色体的核型和银染(Ag-NORs)。结果表明:玛丽鱼具有23对染色体,核型2n=46t,NF=46;Ag-NORs出现在t2和t9的染色体末端,其数目具有多态性,未发现性染色体。本研究结果可为研究玛丽鱼的遗传方式提供基础资料。
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| 关 键 词： | 玛丽鱼 染色体核型 银染 |
Karyotype and Ag-NORs of sailfin molly Poecilia latipinna |
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| Abstract: | The metaphase chromosome spreading was conducted in gills and fins cells of sailfin molly Poecilia latip-inna by colchicine-low concentration-hot film drops, and Ag-NORs were analyzed to obtain cytogenetic data of sailfin molly. The results showed that the karyotype of sailfin molly composed of 2n=46t, NF=46. Ag-NORs were found on the end of chromosome No. 2 and chromosome No. 9, and the Ag-NORs polymorphisms were individually specific, while no sex chromosome was observed in this fish. The findings will provide reference to genetic pattern of sailfin molly. |
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| Keywords: | Poecilia latipinna karyotype Ag-NORs |
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