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邢台野生酸枣组织培养研究
引用本文:石晓云,王僧虎,张雪辉,唐蕊.邢台野生酸枣组织培养研究[J].安徽农业科学,2011,39(13):7607-7608.
作者姓名:石晓云  王僧虎  张雪辉  唐蕊
作者单位:邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001
摘    要:目的]建立邢台野生酸枣组织培养快繁体系,为野生酸枣的规模化开发种植奠定基础。方法]以邢台野生酸枣当年生枝条为外植体进行组培快繁研究,并探讨合适的培养基种类。结果]邢台野生酸枣组织培养外植体最佳消毒方法是浓度70%乙醇消毒30 s,0.1%升汞消毒10 min。其最佳培养基组合为:诱导培养基1/2MS+6-BA1.0 mg/L+NAA0.2 mg/L;增殖培养基MS+6-BA1.0或1.5 mg/L+NAA0.2 mg/L;生根培养基1/2MS+6-BA0.5 mg/L。结论]初步研究出邢台野生酸枣组织培养方法,并筛选出合适的诱导、增殖及生根培养基。

关 键 词:野生酸枣  组织培养  增殖

Studies on Tissue Culture of Xingtai Wild Jujube
SHI Xiao-yun et al.Studies on Tissue Culture of Xingtai Wild Jujube[J].Journal of Anhui Agricultural Sciences,2011,39(13):7607-7608.
Authors:SHI Xiao-yun
Institution:SHI Xiao-yun et al(Department of Biology and Chemistry,Xingtai University,Xingtai,Hebei 054001)
Abstract:Objective] The basis of the industrialization development of wild jujube(Ziziphus jujuba var.spinosa(Bunge)Hu) was laid through the establishment of the multiplication system of its tissue culture.Method] The medium suitable for the wild jujube,in which,the one-year branch was used as explants,was researched and selected.Result] The optimum disinfect way for the explants was with 70% C2H5OH for 30 seconds and then with 0.1% HgCl2 for 10 minutes.The optimum medium combination was: the induction medium was...
Keywords:Wild jujube  Tissue culture  Multiplication  
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