| 2种提取方法测定大野荞植物叶中的黄酮含量 |
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| 引用本文： | 凌永霞,黄凯丰,陈庆富.2种提取方法测定大野荞植物叶中的黄酮含量[J].安徽农业科学,2011(13):7768-7770. |
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| 作者姓名： | 凌永霞 黄凯丰 陈庆富 |
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| 作者单位： | 贵州师范大学生命科学学院植物遗传育种研究所; |
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| 基金项目： | 国家燕麦荞麦产业体系荞麦育种岗位科学家资金项目; 国家自然科学基金项目(31060207); 贵州省农业攻关项目[黔科合NY字(2010)3094]; 贵州省动植物育种专项[黔农育专字(2010)023号] |
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| 摘 要： | 目的]比较2种方法测定荞麦黄酮含量的效果。方法]采用水浴提取和超声提取2种方法,用紫外分光光度计测定大野荞叶中总黄酮含量。结果]测定大野荞植物叶中黄酮含量的最适波长为420 nm,超声提取法和水浴提取法所得大野荞叶中平均总黄酮含量分别为5.26%和5.40%,且水浴提取法所得平均总黄酮含量极显著高于超声提取法。结论]该研究结果对改进荞麦黄酮含量测定方法具有重要意义。
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| 关 键 词： | 大野荞 超声提取法 水浴提取法 黄酮 |
Comparison of Two Methods for Measuring Flavonoid Content of Fagopyrum megaspartanium Leaves |
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| LING Yong-xia et al.Comparison of Two Methods for Measuring Flavonoid Content of Fagopyrum megaspartanium Leaves[J].Journal of Anhui Agricultural Sciences,2011(13):7768-7770. |
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| Authors: | LING Yong-xia |
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| Institution: | LING Yong-xia et al(Institute of Plant Genetics and Breeding,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001) |
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| Abstract: | Objective] The aim was to compare with effects two method to determine the content of flavonoid of Fagopyrum megaspartanium leaves.Method] By using water bath extraction and ultrasonic extraction,the total flavonoid content in buckwheat leaves was measured with UV spectrophotometer.Result] The optimum wavelength for determination of the content of flavonoid extracted from buckwheat leaves was 420 nm;the content of total flavonoid extracted by ultrasonic extraction and water bath extraction from buckwheat... |
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| Keywords: | Fagopyrum megaspartanium Ultrasonic extraction Water bath extraction Flavone |
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