| 新城疫病毒HeB02分离株F基因的序列分析 |
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| 引用本文： | 孙一敏,边艳青,柏佳宁,孙继国,赵宝华.新城疫病毒HeB02分离株F基因的序列分析[J].中国兽医学报,2005,25(1):4-6. |
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| 作者姓名： | 孙一敏 边艳青 柏佳宁 孙继国 赵宝华 |
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| 作者单位： | 1. 河北师范大学,生命科学学院,河北,石家庄,050016
2. 河北农业大学,动物科技学院,河北,保定,071001 |
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| 基金项目： | 河北省重点学科基金资助项目 (2 0 0 10 2 ) |
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| 摘 要： | 根据国外已发表的新城疫病毒F基因序列，设计了1对引物，并以RT-PCR特异性扩增出新城疫病毒HeB02分离株的F基因，基因产物大小为1．63kb，与设计相符。对其进行序列测定后，与其他标准毒株F48E9、LaSota和Clone30的F基因进行了同源性比较，结果表明，HeB02株与国内标准强毒株F48E9及目前广泛应用的弱毒疫苗株LaSota和Clone30的F基因核苷酸序列的同源性分别为88.1％、84.9％和83.8％。由此可以看出，HeB02分离株与标准毒株和疫苗株相比，在F基因上发生了变导。
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| 关 键 词： | F基因 新城疫病毒 分离株 强毒株 弱毒疫苗株 序列分析 引物 基因产物 RT-PCR 扩增 |
| 文章编号： | 1005-4545(2005)01-0004-02 |
| 修稿时间： | 2004年2月16日 |
Nucleotide Sequence Analysis and Expression of the F Gene of NDV Isolate HeB02 |
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| SUN Yi-min,BIAN Yan-qing,BAI Jia-ning,SUN Ji-guo,ZHAO Bao-hua.Nucleotide Sequence Analysis and Expression of the F Gene of NDV Isolate HeB02[J].Chinese Journal of Veterinary Science,2005,25(1):4-6. |
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| Authors: | SUN Yi-min BIAN Yan-qing BAI Jia-ning SUN Ji-guo ZHAO Bao-hua |
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| Institution: | SUN Yi-min~1,BIAN Yan-qing~1,BAI Jia-ning~1,SUN Ji-guo~2,ZHAO Bao-hua~ |
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| Abstract: | One pair of primers for amplifying the F gene of NDV were designed, with which the predicted 1.63 kb fragment had been amplified via RT-PCR from NDV strain HeB02. The F gene of strain HeB02 was sequenced and compared with standard strains such as F_(48)E_9, LaSota and Clone30. The results showed that the homologies of nucleic acids between HeB02 strain and F_(48)E_9, LaSota and Clone30 strains were 88.1%, 84.9% and 83.8%, respectively. The difference of the F gene of NDV strain HeB02 with those of other NDV standard strains had been demonstrated. |
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| Keywords: | Newcastle disease virus F gene nucleotide analysis |
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