| RNA干扰水稻SBE3基因的表达对籽粒淀粉合成及其关键酶活性的影响 |
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| 引用本文： | 汪结明,张建,江海洋,朱苏文,范军,程备久.RNA干扰水稻SBE3基因的表达对籽粒淀粉合成及其关键酶活性的影响[J].作物学报,2010,36(2):313-320. |
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| 作者姓名： | 汪结明 张建 江海洋 朱苏文 范军 程备久 |
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| 作者单位： | 安徽农业大学作物生物学安徽省重点实验室，安徽合肥230036 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目 |
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| 摘 要： | 为探讨RNA干扰水稻SBE3基因的表达对籽粒淀粉合成及其关键酶活性的影响,以水稻品种中花11及以其为受体的转基因株系为材料,分别检测水稻SBE3基因的表达、籽粒发育各时期淀粉合成关键酶活性变化及直链、支链淀粉相对含量。结果表明,导入的SBE3基因RNA干扰结构成功地降低了目的基因的表达,并使其酶活性在籽粒发育各时期均显著降低并提前3d达高峰期,且不同株系间具有差异。同时也使不同发育时期籽粒的ADPG-PPase和SSS及DBE活性均不同程度地显著降低,尤其以SSS和ADPG-PPase活性峰值降幅最大。此外,两个转基因水稻株系各时期籽粒直链淀粉含量均显著高于对照,而其成熟籽粒千粒重却显著降低,直链淀粉含量越高,千粒重越低。
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| 关 键 词： | 水稻 RNA干扰 淀粉分支酶3 淀粉合成 酶活 |
| 收稿时间： | 2009-06-11 |
Effects of RNA Interference of SBE3 Gene Expression on Starch Accumulation and Key Enzymes Activities Involved in Starch Synthesis in Transgenic Rice Grain |
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| WANG Jie-Ming,ZHANG Jian,JIANG Hai-Yang,ZHU Su-Wen,FAN Jun,CHENG Bei-Jiu.Effects of RNA Interference of SBE3 Gene Expression on Starch Accumulation and Key Enzymes Activities Involved in Starch Synthesis in Transgenic Rice Grain[J].Acta Agronomica Sinica,2010,36(2):313-320. |
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| Authors: | WANG Jie-Ming ZHANG Jian JIANG Hai-Yang ZHU Su-Wen FAN Jun CHENG Bei-Jiu |
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| Institution: | Anhui Agricultural University, Anhui Provincial Key Laboratory of Crop Biology, Hefei 230036, China |
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| Abstract: | Rice cultivar Zhonghua 11 (control) and its transgenic line were used as the experimental materials. The expression level of SBE3 gene, some related key enzymes activities, and the contents of amylose and amylopectin at different developmental stages of rice grain were measured. Results showed that the SBE3 gene expression level and the SBE activity were distinctly reduced by RNA interference (RNAi), but a little difference was observed between the two transgenic lines. The peak values of SBE activity reach... |
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| Keywords: | Rice RNA interference Starch branching enzyme 3 Starch accumulation Enzyme activity |
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