| 利用AFLP进行榧树雌雄株鉴定的初步研究 |
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| 引用本文： | 梁丹,周秦,殳燕,曾燕如,喻卫武.利用AFLP进行榧树雌雄株鉴定的初步研究[J].浙江林学院学报,2009,26(1):63-67. |
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| 作者姓名： | 梁丹 周秦 殳燕 曾燕如 喻卫武 |
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| 作者单位： | 浙江林学院,浙江省现代森林培育技术重点实验室,浙江,临安,311300 |
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| 基金项目： | 浙江省重大科技攻关项目，浙江林学院人才基金 |
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| 摘 要： | 为了解决榧树Torreya grandis苗期雌雄株鉴别的问题,利用已建立的扩增片断长度多态（AFLP）体系及T-A克隆测序,就榧树的雌雄株开展了研究。结果从15对引物中用引物对E-AGC/M-CAT得到了一条雌性榧树特异的条带。这条榧树雌性性别特异条带,仅在雌株、雌雄同株的个体中有,雄株中没有,推测榧树雌雄同株由雌性榧树变异而来。GenBank序列比对结果发现,数据库没有与此特异条带相匹配的序列。
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| 关 键 词： | 经济林学 榧树 扩增长度多态 叶 T-A克隆测序 |
| 收稿时间： | 2008-1-31 |
| 修稿时间： | 2008-10-6 |
AFLP-based sexual identification in Torreya grandis |
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| LIANG Dan,ZHOU Qin,SHU Yan,ZENG Yan-ru,YU Wei-wu.AFLP-based sexual identification in Torreya grandis[J].Journal of Zhejiang Forestry College,2009,26(1):63-67. |
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| Authors: | LIANG Dan ZHOU Qin SHU Yan ZENG Yan-ru YU Wei-wu |
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| Institution: | (Key Laboratory for Modem Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin＇an 311300, Zhejiang, China) |
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| Abstract: | Torreya trees can be monoecious or dioecious, cones may be on different branches. To identify young and if they are monoecious, the male and female male and female seedlings of T. grandis, an established AFLP （amplified fragment length polymorphism） analysis protocol along with thymine-adenine （T-A） cloning and sequencing were applied to male and female T. grandis seedlings using 15 pairs of primers. Results showed that the E-AGC/M-CAT marker revealed a band restricted to the female whose sequence was not matched to any sequence from GenBank. This female-specific band was only available in the female, monoecious T. grandis trees. It was inferred that monoecious trees were likely derived from female trees. These results have laid a foundation for subsequent studies. Ch, 6 fig. 12 ref.] |
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| Keywords: | cash forestry Torreya grandis AFLP （amplified fragment length polymorphism） leaf T-A cloning and sequencing |
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