| 福建省番茄灰霉病菌对氟啶胺敏感基线建立及与不同杀菌剂的交互抗性 |
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| 引用本文： | 石妞妞,杜宜新,阮宏椿,甘林,杨秀娟,代玉立,陈福如.福建省番茄灰霉病菌对氟啶胺敏感基线建立及与不同杀菌剂的交互抗性[J].农药学学报,2016,18(4):535-539. |
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| 作者姓名： | 石妞妞 杜宜新 阮宏椿 甘林 杨秀娟 代玉立 陈福如 |
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| 作者单位： | 1.福建省农业科学院 植物保护研究所, 福州 350013 |
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| 基金项目： | 福建省农科院青年人才创新基金（2014CX-15）；福建省科技厅项目（2009N2005） |
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| 摘 要： | 为明确中国福建省番茄灰霉病菌对氟啶胺的敏感性及其与不同杀菌剂的交互抗性，采用菌丝生长速率法测定了106株采自福建省主要番茄产区的番茄灰霉病菌对氟啶胺的敏感性。结果表明，氟啶胺对福建省番茄灰霉病菌菌丝生长的EC50值在0.0037～0.0452 μg/mL之间，平均值为（0.0221 ±0.0098）μg/mL，其敏感性频率分布呈连续单峰曲线，符合正态分布，因此可将该EC50平均值（0.0221±0.0098）μg/mL作为福建省番茄灰霉病菌对氟啶胺的敏感基线，用于其田间抗药性监测。从106株菌株中选择15株对氟啶胺敏感性不同的菌株，测定了其对嘧霉胺、异菌脲、腐霉利和啶氧菌酯的敏感性。结果表明，供试5种杀菌剂对15株番茄灰霉病菌菌丝生长的平均抑制活性依次为氟啶胺 >异菌脲 >腐霉利 >啶氧菌酯 >嘧霉胺，氟啶胺与异菌脲、腐霉利、啶氧菌酯和嘧霉胺之间均不存在交互抗性。
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| 关 键 词： | 番茄灰霉病菌 氟啶胺 敏感基线 交互抗性 |
| 收稿时间： | 3/1/2016 12:00:00 AM |
| 修稿时间： | 2016/4/27 0:00:00 |
Baseline sensitivity of Botrytis cinerea to fluazinam and cross-resistance to different fungicides in Fujian province |
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| SHI Niuniu,DU Yixin,RUAN Hongchun,GAN Lin,YANG Xiujuan,DAI Yuli and CHEN Furu.Baseline sensitivity of Botrytis cinerea to fluazinam and cross-resistance to different fungicides in Fujian province[J].Chinese Journal of Pesticide Science,2016,18(4):535-539. |
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| Authors: | SHI Niuniu DU Yixin RUAN Hongchun GAN Lin YANG Xiujuan DAI Yuli and CHEN Furu |
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| Institution: | 1.Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China2.Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou 350003, China |
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| Abstract: | To evaluate the sensitivity ofBotrytis cinerea to fluazinam and the existed cross-resistance between fluazinam and other fungicides, 106 single spore isolates ofB. cinerea were collected from tomato in Fujian province. The sensitivities ofB. cinerea isolates to fluazinam were determined by mycelial growth rate test. The results showed that EC50 values of 106 isolates to fluazinam followed a unimodal curve, ranging from 0.003 7 to 0.045 2 μg/mL with the mean EC50 value of (0.022 1 ± 0.009 8) μg/mL. Thus, the data could be employed as the baseline-sensitivity ofB. cinerea to fluazinam which could be used for the field resistance monitoring. And the sensitivities of 15 isolates ofB.cinerea, which exhibited different sensitivities to fluazinam, to pyrimethanil, iprodione, procymidone and picoxystrobin, were also tested with the mycelial growth rate method. The mycelial growth inhibition rates of the above mentioned 5 fungicides were fluazinam> iprodione> procymidone>picoxystrobin> pyrimethanil. No cross-resistance between fluazinam and other 4 fungicides was observed. |
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| Keywords: | Botrytis cinerea fluazinam baseline sensitivity cross-resistance |
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