Characterization and Evaluation of OsLCT1 and OsNramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice |
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| Authors: | Liu Songmei Jiang Jie Liu Yang Meng Jun Xu Shouling Tan Yuanyuan Li Youfa Shu Qingyao Huang Jianzhong |
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| Institution: | 1. National Key Laboratory of Rice Biology/Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310058, China;2. Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434025, China;3. Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment/College of Environmental and Natural Resource Sciences, Zhejiang University, Hangzhou 310058, China;4. Jiaxing Academy of Agricultural Sciences, Jiaxing 314016, China |
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| Abstract: | To explore how rice (Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant (lct1×1) and two of OsNramp5 mutants (nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40% (0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard (0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation (< 0.06 mg/kg) in the paddy (approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. |
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| Keywords: | cadmium rice genome-editing heavy metal contamination CRISPR Cas9 |
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