| 香蕉叶片微管免疫荧光染色方法的建立与应用 |
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| 引用本文： | 乔飞,曹晶潇,江雪飞,徐立.香蕉叶片微管免疫荧光染色方法的建立与应用[J].热带作物学报,2012,33(8):1409-1411. |
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| 作者姓名： | 乔飞 曹晶潇 江雪飞 徐立 |
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| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所 农业部华南作物基因资源与种质创制重点开放实验室 海南儋州571737
2. 海南大学园艺园林学院,海南海口,570228 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金（No. PZS079）；农业部热带作物种质资源利用重点开放实验室开放基金项目（No. KFKT-2010-05）。 |
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| 摘 要： | 以香蕉幼叶为材料,通过优化间接荧光免疫染色流程,建立了香蕉微管显微观察方法.结果如下:在微管稳定缓冲溶液中加入37g/L的多聚甲醛,10 mL/L的Triton x-100,10 mL/L的甘油以及10 mL/L的DMSO作为固定液,固定60 min,可以保持微管骨架的原有形态;水解细胞壁30 min,可使抗体有效通过细胞壁.进一步用镰刀菌酸处理香蕉叶片后发现:微管骨架出现解聚和断裂,呈现出典型的抗性响应特征,暗示微管骨架参与了香蕉和枯萎病病原镰刀菌的互作.
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| 关 键 词： | 香蕉 微管 荧光免疫染色 细胞骨架 镰刀菌酸 |
Improved Method of Microtubule Cytoskeleton Immunofluorescence for Banana Leaf and Its Appliance |
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| QIAO Fei,CAO Jingxiao,JIANG Xuefei and XU Li.Improved Method of Microtubule Cytoskeleton Immunofluorescence for Banana Leaf and Its Appliance[J].Chinese Journal of Tropical Crops,2012,33(8):1409-1411. |
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| Authors: | QIAO Fei CAO Jingxiao JIANG Xuefei and XU Li |
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| Institution: | Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,;College of Horticulture and Landscape Architecture, Hainan University;College of Horticulture and Landscape Architecture, Hainan University;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, |
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| Abstract: | A staining method was optimized to visualize the microtubule cytoskeleton of banana leaf.It was revealed that 60 min fixation with fixative containing 3.7% paraformadehyde, 1% trixon x-100, 1% glycerol and 1% DMSO was suitable for maintaining the microtubule organization; and 30 min digestion could facilitate antibody penetrating cell wall; further, the response of microtubule was observed when challenged by fusaric acid; and microtubule underwent fragmenting,indicating that cytoskeleton was involved in the interaction between banana cell and Fusarium oxysporum f. sp. cubense. |
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| Keywords: | Bbanana Microtubule Immunofluorescence Cytoskeleton Fusaric acid |
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