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规模化牛场奶牛衣原体和布鲁菌病相关抗体的检测分析
引用本文:邱洪流,谢琴.规模化牛场奶牛衣原体和布鲁菌病相关抗体的检测分析[J].动物医学进展,2006,27(7):91-93.
作者姓名:邱洪流  谢琴
作者单位:1. 宁夏医学院护理学院,宁夏银川,750021
2. 宁夏大学生命科学学院,宁夏银川,750021
摘    要:为检测并分析银川地区规模化牛场牛衣原体和布鲁菌病的相关抗体,采用鹦鹉热衣原体McAb-ELISA方法和布鲁菌虎红平板凝集试验(RBPT)方法进行特异性检测,应用McAb-ELISA方法与传统的IHA试验方法分别对同样50份待检牛血清进行衣原体抗体的比较检测。结果表明,银川地区的16个牛场1 161份牛血清衣原体总的阳性率为9.22%。布鲁菌的血清阳性率为13.26%。对50份牛血清采用2种试验方法同时进行衣原体抗体检测,ELISA阳性检出率为32%,IHA为24%。McAb-ELISA比IHA的特异性强。

关 键 词:鹦鹉热衣原体  布鲁菌病  血清  人兽共患病
文章编号:1007-5038(2006)07-0091-03
收稿时间:2006-03-27
修稿时间:2006年3月27日

Detection of Antibodies Associated with Chlamydia and Brucella Infections in Large Scale Cow Farms
QIU Hong-liu,XIE Qin.Detection of Antibodies Associated with Chlamydia and Brucella Infections in Large Scale Cow Farms[J].Progress In Veterinary Medicine,2006,27(7):91-93.
Authors:QIU Hong-liu  XIE Qin
Institution:1. College of Nursing ,Ningxia Medical College, Yinchuan ,Ningxia, 750021, China; 2. Life Science College, Ningxia University, Yinchuan , Ningxia, 750021, China
Abstract:In order to detect the antibodies associated with Chlamydia and Brucella infection in large scale cow farms in Yinchuan region,Chlamydia McAb-ELISA,IHA and Brucella RBPT were used.The results showed that totall positive rates of Chlamydia and Brucella in fections in 1 161 seral samples were 9.22% and 13.26%,respectively.McAb-ELISA and IHA were used for detecting Chlamydia antibodies in 50 seral samples simutaneously,the positive rates were 32% and 24%,respectively.The results illustrated that McAb-ELISA was more sensitive than IHA in detecting Chlamydia infection in cows.
Keywords:Chlamydia psittaci  Brucella  serum  zoonoses
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