| 青萝卜游离小孢子培养体系优化 |
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| 引用本文： | 张胜雪,范伟强,王超楠,张红,黄志银,李梅,刘晓晖,尹婧,张斌.青萝卜游离小孢子培养体系优化[J].中国瓜菜,2022(2). |
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| 作者姓名： | 张胜雪 范伟强 王超楠 张红 黄志银 李梅 刘晓晖 尹婧 张斌 |
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| 作者单位： | 天津师范大学生命科学学院;天津科润蔬菜研究所·蔬菜种质创新国家重点实验室·天津市蔬菜遗传育种企业重点实验室;天津市农业科学院蔬菜研究所 |
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| 基金项目： | 财政部和农业农村部:国家现代农业产业技术体系(CARS-23-G-07);天津市(蔬菜)现代农业产业技术体系创新团队(ITTVRS2021003)。 |
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| 摘 要： | 以12份不同基因型青萝卜品种为供试材料,采用游离小孢子培养技术,研究基因型、培养基类型、激素浓度配比、4℃冷处理时间及32.5℃热激处理时间对青萝卜小孢子胚诱导的影响,为青萝卜高频胚诱导提供参考依据,从而完善青萝卜游离小孢子培养技术体系。结果显示,在相同培养条件下,只有SX1、SX8与SX11三份材料获得了胚状体,其余材料未获得胚状体,且不同供试材料间小孢子胚诱导率差异显著,表明基因型是决定青萝卜小孢子胚诱导成功的关键因素之一;利用1/2NLN-13型培养基配套指定激素配比(0.2 mg·L-16-BA+1 mg·L-1 NAA)培养时,材料SX1、SX8与SX11小孢子出胚率最高,基本确定了青萝卜小孢子培养的最适培养基类型及最佳激素浓度组合;此外,4℃冷处理1 d和32.5℃热激处理2 d,材料SX1与SX11小孢子出胚率最高,确定了小孢子胚诱导最佳冷处理和热激处理条件。
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| 关 键 词： | 青萝卜 小孢子培养 胚状体 优化 |
Optimization of isolated microspore culture system in green radish |
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| ZHANG Shengxue,FAN Weiqiang,WANG Chaonan,ZHANG Hong,HUANG Zhiyin,LI Mei,LIU Xiaohui,YIN Jing,ZHANG Bin.Optimization of isolated microspore culture system in green radish[J].China Cucurbits And Vegetables,2022(2). |
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| Authors: | ZHANG Shengxue FAN Weiqiang WANG Chaonan ZHANG Hong HUANG Zhiyin LI Mei LIU Xiaohui YIN Jing ZHANG Bin |
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| Institution: | (College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;State Key Laboratory of Vegetable Germplasm Innovation/Tianjin Key Laboratory of Vegetable Genetics and Breeding Enterprises/Tianjin Kerun Vegetable Research Institute,Tianjin 300381,China;Vegetable Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China) |
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| Abstract: | Twelve genotypes of Green Radish were tested for isolating microspore culture technology.The effects of genotype,medium type,growth regulator,4℃ cold treatment and 32.5℃ heat shock treatment on the embryo induction from microspores were studied to improve isolated microspore culture technology system for green radish.The results showed that under the same culture conditions,only three materials SX1,SX8 and SX11 produced embryos,and the embryos induction rate from microspores was different among materials tested.Genotype is one of the key factors determining the success of embryo induction from green radish microspores;When 1/2 NLN-13 medium supplemented with 0.2 mg·L-16-BA+1 mg·L-1 NAA genotypes SX1,SX8 and SX11 produced highest number of embryos.In addition,of 4℃cold treatment for 1 d and 32.5℃heat shock treatment for 2 d SX1 and SX11 had the highest embryo rate from microspores.Our research generated an optimized protocol of generating embryos from isolated microspores of green radish. |
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| Keywords: | Green radish Microspore culture Embryoid Optimization |
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