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谷氨酰胺转氨酶对不同脂肪含量Cheddar干酪品质的影响
引用本文:周炜,胡亚男,吕新,任发政.谷氨酰胺转氨酶对不同脂肪含量Cheddar干酪品质的影响[J].中国奶牛,2013(19):56-61.
作者姓名:周炜  胡亚男  吕新  任发政
作者单位:[1]北京首都农业集团有限公司,北京100029 [2]中国农业大学食品学院,食品质量与安全北京实验室,北京100083
基金项目:基金项目十二五”国家科技支撑计划项目“新型乳制品的研发与产业化示范”(2012BAD28807);‘‘十二五”农村领域国家科技计划‘‘传统发酵乳制品微生物资源开发与产业化关键技术”(2011AA100903).
摘    要:本研究在添加凝乳酶的同时,添加谷氨酰胺转氨酶(TG)(1.0U/g蛋白),测定不同脂肪含量Cheddar干酪的得率和组分回收率,并监测成熟90d中干酪理化、功能指标的变化以及蛋白水解情况,分析TG对不同脂肪含量Cheddar干酪品质的改善作用..结果表明,添加TG的全脂干酪的得率、蛋白和脂肪回收率与对照组相比差别不大,添加TG的减脂干酪的得率、蛋白和脂肪回收率比对照组分别增加2.1%、1.9%和13G,(P〈0.05),添加TG的低脂干酪的得率、蛋白和脂肪回收率比对照组分别增加2.2%、7%和12%(P〈0,05)、干酪成熟前期(1~15d),添加TG酶的减脂、低脂干酪的硬度显著低于对照组(P〈0.05),而全脂干酪变化不明显?随着成熟期的延长,TG酶对酪蛋白的交联作用抑制了蛋白的水解,导致干酪硬度增大。

关 键 词:谷氨酰胺转氨酶  Cheddar干酪  流变  质构

Effects of Transglutaminase on the Properties of Cheddar Cheese with Different Fat Content
ZHOU Wei,HU Ya-nan,LU Xin,REN Fa-zheng.Effects of Transglutaminase on the Properties of Cheddar Cheese with Different Fat Content[J].China Dairy Cattle,2013(19):56-61.
Authors:ZHOU Wei  HU Ya-nan  LU Xin  REN Fa-zheng
Institution:1. Beijing Capital Agribusiness Group, Beijing 100029; 2. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Bijing 100083)
Abstract:To study the effects of TG on the yield, composition, proteolysis and textural properties of Cheddar cheese with different fat content, TG was added at a concentration of 1.0 U/g protein simultaneously with the rennet. By adding TG, the yield, protein and fat recoveries of reduced-fat cheese were increased by 2.1%, 1.9% and 13% significantly (P〈0.05), and low-fat cheese increased by 2.2%, 7% and 12% significantly (P〈0.05). There was no significant change for full-fat cheese. During early ripening (1-15 days), the hardness was reduced significantly (P〈0.05) for both reduced-fat and low-fat cheeses, the hardness for full-fat cheese was not significantly reduced. While the additional covalent cross-linking catalyzed by TG had a slight adverse effect on the protein proteolysis at later ripening days, this resulted in a harder texture than the control cheeses.
Keywords:Transglutaminase  Cheddar cheese  Rheology  Texture
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