| 芥菜转基因再生体系的建立 |
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| 引用本文： | 杨朝辉,雷建军,曹必好,钟小林,李蓉芬,江渝,彭家和,何凤田.芥菜转基因再生体系的建立[J].作物学报,2004,30(5):496-501. |
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| 作者姓名： | 杨朝辉 雷建军 曹必好 钟小林 李蓉芬 江渝 彭家和 何凤田 |
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| 作者单位： | 第三军医大学生物化学与分子生物学教研室，重庆400038 |
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| 摘 要： | 以叶用和茎用芥菜的子叶、叶片及下胚轴为外植体，通过筛选12种含不同种类和浓度激素的培养基，综合分析与确定影响芥菜转基因再生体系各因素的最佳值，以及对再生抗性苗的PCR、Southern、Northern及抗虫鉴定，筛选到了能稳定表达外源基因的转基因植株，建立了芥菜转基因再生体系。
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| 关 键 词： | 转基因 再生 芥菜 |
| 收稿时间： | 2003-07-11 |
| 修稿时间： | 2003年7月11日 |
Establishment of Genetic Transformation Regeneration System in Brassica juncea |
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| YANG Zhao-Hui,LEI Jian-Jun,CAO Bi-Hao,ZHONG Xiao-Lin,LI Rong-Fen,JIANG Yu,PENG Jia-He,HE Feng-Tian.Establishment of Genetic Transformation Regeneration System in Brassica juncea[J].Acta Agronomica Sinica,2004,30(5):496-501. |
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| Authors: | YANG Zhao-Hui LEI Jian-Jun CAO Bi-Hao ZHONG Xiao-Lin LI Rong-Fen JIANG Yu PENG Jia-He HE Feng-Tian |
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| Institution: | Department of Biochemistry, Third Military Medical University, Chongqing 400038 |
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| Abstract: | Using hypocotyls, cotyledons and leaves as explants, 12 sorts of media containing different kinds and concentrations of hormones were studied to establish an efficient regeneration system. The optimum values of the factors affected mustard transgenetic regeneration system were determined. The regenerated plants were identified by PCR, Southern, Northern blot and insect-resistance test. The target plants with cpti gene activity were separated from the Kan-resistant regenerated plants. Therefore the perfect transgenetic regeneration system of mustard was established. |
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| Keywords: | Transgenetic Regeneration Brassica juncea Coss |
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