| 桃芽变品种的RAPD分析 |
| |
| 引用本文： | 郭传翔,潘海发,徐义流,张金云,高正辉,束冰,伊兴凯.桃芽变品种的RAPD分析[J].中国农学通报,2012,28(31):175-180. |
| |
| 作者姓名： | 郭传翔 潘海发 徐义流 张金云 高正辉 束冰 伊兴凯 |
| |
| 作者单位： | 1. 安徽农业大学生命科学学院,合肥,230036
2. 安徽省农业科学院园艺研究所,合肥,230031 |
| |
| 基金项目： | 安徽省水果产业技术体系(皖农科[2011]6号);财政部现代农业发展资金项目[皖农农(2011)359号] |
| |
| 摘 要： | 为了从遗传学基础上对9个桃品种进行分类及亲缘关系研究。利用随机扩增多态性DNA(RAPD)技术对其基因组DNA进行标记，并应用SPSS11.0软件进行聚类分析。聚类分析结果表明：不同桃品种在欧氏距离平方系数阈值为2.0~6.0时，聚为两大类，第一大类由‘满园红’、‘中油4号’、‘中油9号’和07-9-6组成，均为油桃；第二大类由97-6-83、NJC83、‘皖83’、NJC19和07-9-19组成，均为黄桃。在欧氏距离平方系数阈值为1.0~2.0时，所有品种聚为4类，‘NJC83’和其营养系变异97-6-83、‘皖83’聚为一类，NJC19和其变异07-9-19聚为一类，‘中油4号’和其营养系变异‘满园红’聚为一类，‘中油9号’和其变异09-6-9聚为一类。RAPD分析认为：母本与营养系变异间的遗传距离短，具有很近的亲缘关系，其在DNA水平上产生了一定量的变异性条带，出现了较大范围的变异。
|
| 关 键 词： | 酸性磷酸酶 酸性磷酸酶 |
| 收稿时间： | 2012/5/21 0:00:00 |
| 修稿时间： | 2012/5/28 0:00:00 |
RAPD Analysis on Peach Buds Change of Varieties |
| |
| Guo Chuanxiang
,
Pan Haifa
,
Xu Yiliu
,
Zhang Jinyun
,
Gao Zhenghui
,
Shu Bing
,
Yi Xingkai.RAPD Analysis on Peach Buds Change of Varieties[J].Chinese Agricultural Science Bulletin,2012,28(31):175-180. |
| |
| Authors: | Guo Chuanxiang Pan Haifa Xu Yiliu Zhang Jinyun Gao Zhenghui Shu Bing Yi Xingkai |
| |
| Institution: | 1 Life Science Department,Anhui Agricultural University,Hefei 230036;2 Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031) |
| |
| Abstract: | In order to classify the nine peach varieties and research the genetic relationship from the genetic basis,random amplified polymorphic DNA（RAPD） technology was used to mark the genomic DNA and the cluster analysis was performed with SPSS 11.0.The results of cluster analysis showed that when the value of Squared Euclidean distance coefficient was from 2.0 to 6.0,the different peach varieties were divided into two groups.The first group was made up of ＇ Manyuanhong ＇,＇ Zhongyoutao 4 ＇,＇ Zhongyoutao 9 ＇ and 07-9-6.They were all considered as nectarine.The second group was made up of 97-6-83,NJC83,＇ Wan83 ＇,NJC19 and 07-9-19.They were all considered as yellow peach.All the varieties were divided into four groups when the value of Squared Euclidean distance coefficient was from 1.0 to 2.0.NJC83 and its clone mutants（97-6-83 and ＇ Wan83 ＇） were in the same group.NJC19 and its variation（07-9-19） got together.＇ Zhongyoutao 4 ＇ and its clone mutant（＇ Manyuanhong ＇） gathered together.＇ Zhongyoutao 9 ＇ and its variation（09-6-9） clustered together.The RAPD analysis indicated that the genetic distance between the female parent and its mutant was short.They had very close relationship.They have produced a certain amount of specificity strips and also emerged a wide range of variation. |
| |
| Keywords: | peach RAPD mark cluster analysis bud mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
