| 利用solexa测序技术发掘木薯microRNAs |
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| 引用本文： | 张何芳,陈新,卢诚,章伟雄,王文泉.利用solexa测序技术发掘木薯microRNAs[J].中国农学通报,2012,28(30):184-190. |
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| 作者姓名： | 张何芳 陈新 卢诚 章伟雄 王文泉 |
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| 作者单位： | 1. 海南大学,海口570228;中国热带农业科学院热带生物技术研究所,海口571101
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 973计划项目“重要热带作物木薯品种改良的基础研究”(S2009011002) |
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| 摘 要： | 为了发掘木薯中的microRNAs (miRNAs)，了解其在木薯中的调控机制，本研究利用Solexa测序技术在木薯中发现了大量的miRNAs，并利用一种新的miRNA定量检测技术（Multiplexed RT法）对其进行实验验证。通过对构建的3个小RNA文库进行solexa测序与生物信息学分析，最终获得19个家族93个保守的miRNA和74个新的miRNA，实验验证了测序获得的83个miRNA。在Arg7块根中特异表达的有6个miRNA，分别为miR172d、miR396c、miR398a、miR398b、miR399e、miR399f，叶片中特异表达的有13个，须根中特异表达的有3个，这些miRNAs将为深入了解木薯块根发育和淀粉累积机理提供了一个新的视角。
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| 关 键 词： | 聚类分析 聚类分析 |
| 收稿时间： | 3/9/2012 12:00:00 AM |
| 修稿时间： | 2012/4/24 0:00:00 |
Identification of miRNAs in Cassava by Solexa Sequencing |
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| Zhang Hefang
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Chen Xin
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Lu Cheng
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Zhang Weixiong
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Wang Wenquan.Identification of miRNAs in Cassava by Solexa Sequencing[J].Chinese Agricultural Science Bulletin,2012,28(30):184-190. |
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| Authors: | Zhang Hefang Chen Xin Lu Cheng Zhang Weixiong Wang Wenquan |
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| Institution: | 1 Hainan University,Haikou 571737;2 The Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 570028) |
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| Abstract: | The aims were to identify miRNA （microRNA） in Cassava, and understand its mechanism of accumulation in cassava. In this study, lots of miRNAs were identificated in Cassava by Solexa sequencing. By using a new PCR based assay platform （Multiplexed RT assay）, miRNAs were verified in cassava. 93 conserved miRNAs comprising 19 families and 74 novel miRNAs were verified through sequencing and bioinformaties analysing three small RNA library, 83 miRNAs were verified by Multiplexed RT assay. 6 of miRNA, miR172d, miR396c, miR398a, miR398b, miR399e and miR399f, were specifically expressed in Arg7 tuber; 13 in leaves; and 3 in fibrous roots. These miRNAs provided a new perspective to intensive study tuber development and the mechanism of starch accumulation in the cassava. |
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| Keywords: | cassava miRNA Solexa sequencing |
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