| 麻花鸡副黏病毒ZH-1株F基因真核表达载体的构建 |
| |
| 引用本文： | 李红丽,詹丽娥,乔忠,王彩先,陆冰洋.麻花鸡副黏病毒ZH-1株F基因真核表达载体的构建[J].华北农学报,2009,24(3). |
| |
| 作者姓名： | 李红丽 詹丽娥 乔忠 王彩先 陆冰洋 |
| |
| 作者单位： | 山西省农科院畜牧兽医研究所,山西,太原,030032 |
| |
| 基金项目： | 山西省自然科学基金资助项目 |
| |
| 摘 要： | 将麻花鸡副黏病毒ZH-1株毒种接种于11日龄SPF鸡胚,收集48～96h死亡的鸡胚尿囊液.参考已发表的鸡源副黏病毒F基因序列,设计并合成了一对特异性引物,用以扩增麻花鸡副黏病毒ZH-1株F基因,预期扩增的F基因片段包含完整的开放阅读框.通过RT-PCR扩增出麻花鸡副黏病毒ZH-1株F基因片段,琼脂糖凝胶电泳回收、纯化,得到F基因片段,经EcoR I和Sal I消化,将F基因克隆进入PCI-neo载体,转化大肠杆菌DHSa,挑选菌落,经限制性核酸内切酶Sal I和EcoR I双酶切及PCR鉴定,结果证明重组真核表达载体PCI-neo-F构建成功,为下一步在哺乳动物细胞vero细胞中表达打下良好的基础.扩增的F基因测序后,与其他禽副黏病毒1型F基因进行系统发育树的分析比较,为阐明麻花鸡副黏病毒ZH-1株的遗传背景奠定了基础.
|
| 关 键 词： | 麻花鸡副黏病毒 F基因 序列分析 重组质粒 |
Construction of the F Gene Eukaryotic Expression Vector of the Paramyxovirus Strain ZH-1 From Brambling |
| |
| LI Hong-li,ZHAN Lie,QIAO Zhong,WANG Cai-xian,LU Bing-yang.Construction of the F Gene Eukaryotic Expression Vector of the Paramyxovirus Strain ZH-1 From Brambling[J].Acta Agriculturae Boreali-Sinica,2009,24(3). |
| |
| Authors: | LI Hong-li ZHAN Lie QIAO Zhong WANG Cai-xian LU Bing-yang |
| |
| Institution: | Institute of Animal Husbandry and Veterinavy;Shanxi Academy of Agricultural Sciences;Taiyuan 030032;China |
| |
| Abstract: | The allantoic fluid was collected from the dead chicken embryo within 48-96 hours after the 11-day-old SPF brambling chicken embryos by type ZH-1.To amplify the sequence of the fusion gene(F-gene)and to determine whether the amplified sequence contains the integrated sequence of open reading frame(ORF),one pair of specific primer was designed and synthesized based on the published sequence of the F gene and then was used to amplify the F-gene in brambling by RT-PCR.The amplified fragment was taken back and ... |
| |
| Keywords: | Paramyxovirus of brambling chicken F gene Sequence analysis Recombinant plasmid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
