| 荧光实时定量PCR技术及其在水产养殖研究中的应用 |
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| 引用本文： | 任伟成,王崇明,李赟.荧光实时定量PCR技术及其在水产养殖研究中的应用[J].海洋水产研究,2007,28(6):97-103. |
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| 作者姓名： | 任伟成 王崇明 李赟 |
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| 作者单位： | 1. 中国海洋大学,青岛,266003
2. 中国水产科学研究院黄海水产研究所,青岛,266071 |
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| 基金项目： | 国家高技术研究与发展项目(2006AA100307)，山东省科技攻关项目(2004GG2205115) |
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| 摘 要： | 荧光实时定量PCR技术是近年来快速发展起来的一门新技术,它是核酸探针技术、荧光共振能量传递技术(Fluorescence Resonance Energy Transfer,FRET)和PCR技术的有机结合。与常规PCR相比,它具有特异性更强、有效解决PCR污染问题、自动化程度高、能较准确定量等特点。本文综述了荧光实时定量PCR技术的基本原理及几种广泛应用的荧光化学方法,并概述了荧光实时定量PCR技术在水产养殖研究领域的应用现状,并对荧光实时定量PCR技术的应用前景进行了展望。
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| 关 键 词： | 荧光实时定量PCR 水产养殖 |
| 文章编号： | 1000-7075（2007）06-0097-07 |
| 收稿时间： | 2006-08-22 |
| 修稿时间： | 2006-11-01 |
Real-time fluorescent quantitative PCR technique and its application in aquaculture-A review |
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| REN Wei-cheng,WANG Chong-ming,LI Yun.Real-time fluorescent quantitative PCR technique and its application in aquaculture-A review[J].Marine Fisheries Research,2007,28(6):97-103. |
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| Authors: | REN Wei-cheng WANG Chong-ming LI Yun |
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| Abstract: | Real-time fluorescent quantitative PCR(Real-time PCR)technique had been under rapid development in recent years.As an integration of fluorescent probes,fluorescence resonance energy transfer(FRET)and traditional PCR techniques,real-time PCR exceeded the limitations of traditional PCR method by allowing for high sensibility,specificity and automa- tion,and avoiding pollution in traditional PCR.This review covers the basic principles and sev- eral fluorescent techniques used widely in real-time fluorescent quantitative PCR,and the cur- rent applications of this technique in aquaculture.The disadvantage and future prospect of appli- cations for real-time PCR were discussed. |
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| Keywords: | Real-time PCR Aquaculture |
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