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| 硫素对不同基因型大豆球蛋白组成及含量的影响 | |
| 引用本文： | 李子靖,蔡柏岩,接伟光,刘潇睿,王丽阳,葛菁萍.硫素对不同基因型大豆球蛋白组成及含量的影响[J].中国农学通报,2012,28(6):53-57. |
| 作者姓名： | 李子靖 蔡柏岩 接伟光 刘潇睿 王丽阳 葛菁萍 |
| 作者单位： | 1. 黑龙江大学生命科学学院/分子生物学重点实验室,哈尔滨,150080 2. 黑龙江大学生命科学学院/分子生物学重点实验室,哈尔滨150080;黑龙江东方学院,哈尔滨150086 3. 黑龙江东方学院,哈尔滨,150086 |
| 基金项目： | 国家自然科学基金资助项目“连作大豆土壤AM真菌菌群结构分析及其与根腐病病原菌相互关系的研究”（31170466）;黑龙江省自然科学基金资助项目“硫有效性对大豆菌根侵染的调控及其与硫素代谢和产质量关系的研究”（C200918）;黑龙江省博士后科研启动金资助项目“硫有效性对大豆菌根侵染的调控及其与硫素代谢和产质量关系的研究”（LBH-Q09022）;黑龙江大学高层次人才支持计划项目“生态修复团队”（Hdtd2010-12）;黑龙江省教育厅科学技术研究（指导）项目“AM真菌与重迎茬大豆产质量关系的研究”（11553080） |
| 摘 要： | 为了探讨施硫量对不同基因型大豆7S和11S球蛋白亚基组成及含量的影响，寻找不同基因型大豆品种最佳施硫水平，以期提高大豆籽粒球蛋白的含量，改善期品质。选用在黑龙江省种植面积较大的‘黑农48’（高蛋白）、‘黑农37’（中间型）和‘黑农44’（高油）3种大豆作为试验材料。采用盆栽种植，每个品种设S1、S2、S3、S4 4个S处理（即每千克土壤分别施单质硫0、0.02、0.04、0.06 g）。采用SDS-PAGE凝胶电泳方法，对成熟大豆籽粒球蛋白亚基组成和含量2个指标进行研究。结果表明：在3个大豆品种中，7S球蛋白由α′、α、γ、β 4个亚基组成；11S球蛋白由酸性亚基和碱性亚基组成；各种亚基分子量在品种间的差异不显著。同一品种不同处理中均以S3水平下蛋白含量最高，对不同品种施用硫肥时，以高油品种提高球蛋白含量效果显著。施用硫肥对3个大豆品种的7S和11S球蛋白各种亚基组成都没有影响，对分子量的大小影响很小。施用硫肥对3个大豆品种中的7S和11S球蛋白和亚基含量有显著影响，适宜的施用硫肥有利于提高各品种球蛋白和亚基的含量。 |
| 关 键 词： | 气候变化 农业气象灾害 本溪山区 气候变化 农业气象灾害 本溪山区 |
| 收稿时间： | 2011/9/18 0:00:00 |
| 修稿时间： | 2011/11/11 0:00:00 |
Effect of Sulfur on the Composition and Accumulation of Globulin in Different Soybean Varieties |
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| Li Zijing , Cai Baiyan , Jie Weiguang , Liu Xiaorui , Wang Liyang , Ge Jingping.Effect of Sulfur on the Composition and Accumulation of Globulin in Different Soybean Varieties[J].Chinese Agricultural Science Bulletin,2012,28(6):53-57. | |
| Authors: | Li Zijing Cai Baiyan Jie Weiguang Liu Xiaorui Wang Liyang Ge Jingping |
| Institution: | 1(1Key Laboratory of Molecular Biology/College of Life Sciences,Heilongjiang University,Harbin 150080;2Heilongjiang East University,Harbin 150086) |
| Abstract: | In order to evaluate the effect of sulfur on the composition and accumulation of 7S and 11S globulin subunits,the best phosphorus levels of different genotype soybean varieties were explored to increase the content of dietary fiber and improve their qualities.Three soybean varieties ’（Heinong 48’,high-protein cultivar;’Heinong 37’,middle cultivar;and’Heinong 44’,high-oil cultivar） grown more in Heilongjiang Province were investigated.The soybean seeds were planted in pots.Four levels of sulfur（S） treatment were designed,which were S1,S2,S3 and S4（0,0.02,0.04,0.06 g of sulfur per kilogram of soil）.The composition and accumulation of mature soybean seed proteins was determined by conducting SDS-PAGE.It showed that：the 7S globulin comprised of 4 subunits of α’,α,γ,and β;and 11S globulin comprised of acidic and basic subunits in the three soybean varieties.No significant differences were observed between the varieties with regard to the molecular weights of the globulin subunits.The concentration of the globulins in the same variety was the highest under the S3 treatment,while they were the highest in high-oil cultivar in different soybean varieties.S fertilization has no effect on the 7S and 11S globulin subunits of soybean cultivars and has only a slight effect on the molecular weights of these subunits.Significant differences were observed in the concentrations of 7S and 11S globulin subunits with the S-nutrition in the three varieties.These findings suggest that appropriate amounts of S fertilization might contribute to the increased concentrations of both soybean globulins and their subunits. |
| Keywords: | soybean seed sulfur globulin subunits composition accumulation |
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