| 春兰名品杂交后代快繁与分化研究 |
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| 引用本文： | 孙芳,李承秀,张林,袁利利,于永畅,牛田,王长宪.春兰名品杂交后代快繁与分化研究[J].中国农学通报,2012,28(10):189-193. |
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| 作者姓名： | 孙芳 李承秀 张林 袁利利 于永畅 牛田 王长宪 |
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| 作者单位： | 1. 山东农业大学林学院,山东泰安,271018
2. 泰山林业科学院,山东泰安,271000 |
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| 基金项目： | 国家公益专项“国兰新品种培育与产业化生产关键技术研究”（201004080） |
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| 摘 要： | 为了解决采用传统方法繁殖春兰速度慢的问题,对春兰名品‘集圆’与‘如意素’杂交获得的根状茎进行增殖与分化研究。设置不同的基本培养基、6-BA、NAA、IBA,探讨其对根状茎增殖与分化的影响。结果表明,1/2MS为春兰杂交根状茎的最适培养基;最佳增殖培养基为1/2MS+NAA 5.0 mg/L+6-BA0.5 mg/L,1/2MS+NAA 0.5 mg/L+6-BA 2.0 mg/L对根状茎的分化效果最好。组培快繁与分化是加快新品种培育的重要方法与手段。
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| 关 键 词： | 春兰 根状茎 增殖 分化 |
| 收稿时间： | 2012/1/12 0:00:00 |
| 修稿时间： | 2012/2/27 0:00:00 |
Study on Rapid Propagation and Differentiation of Filial Generation of Cymbidium goeringii |
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| Sun Fang
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Li Chengxiu
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Zhang Lin
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Yuan Lili
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Yu Yongchang
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Niu Tian
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Wang Changxian.Study on Rapid Propagation and Differentiation of Filial Generation of Cymbidium goeringii[J].Chinese Agricultural Science Bulletin,2012,28(10):189-193. |
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| Authors: | Sun Fang Li Chengxiu Zhang Lin Yuan Lili Yu Yongchang Niu Tian Wang Changxian |
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| Institution: | 1 College of Forestry,Shandong Agricultural University ,Tai＇an Shandong 271018; 2 Forestry Research Institute of Mount ,Tai＇an Shandong 271000) |
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| Abstract: | The aim was to study the rapid propagation and differentiation of rhizomes of Cymbidium goeringii ＇Jiyuan＇ and ＇Ruyisu＇ .Four factors including basic medium,6-BA,NAA and IBA were designed to study the effects on rapid propagation and differentiation of rhizomes.The most appropriate cultivating medium was 1/2MS.1/2MS ＋ NAA 5.0 mg/L ＋ 6-BA 0.5 mg/L was the best propagation medium and the rhizomes differentiates in high rate on 1/2MS＋NAA 0.5 mg/L ＋6-BA 2.0 mg/L.Propagation and differentiation in vitro culture were the important means and methods for speeding up the cultivation of new varieties. |
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| Keywords: | Cymbidium goeringii rhizome multiplication differentiation |
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