| 白皮松组织培养研究 |
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| 引用本文： | 张利,闫辉,高文强,赵红霞,樊金会.白皮松组织培养研究[J].中国农学通报,2012,28(7):13-16. |
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| 作者姓名： | 张利 闫辉 高文强 赵红霞 樊金会 |
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| 作者单位： | 山东农业大学林学院,山东泰安,271018 |
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| 基金项目： | 山东省科技发展计划项目“白皮松抗盐碱性的遗传改造”（2009GG10009009）; 泰安市大学生科技创新行动计划项目“白皮松多倍体诱导”（2010D2007） |
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| 摘 要： | 为建立高效的再生体系，以白皮松成熟胚，子叶为外植体，经过初代培养和继代增殖培养，研究了不同植物生长调节剂种类及浓度、活性炭、GA3在组培各阶段的作用。结果表明：适合子叶诱导芽的培养基为MS+6-BA 3 mg/L+NAA 0.1 mg/L+TDZ 0.01 mg/L，适合胚诱导芽的培养基为MS+6-BA 2 mg/L+NAA 0.1 mg/L；适宜的继代培养基为MS+NAA 0.05 mg/L；以预处理胚（切除下胚轴）为外植体适宜生根培养基为1/2MS+IBA 0.3 mg/L；活性炭对芽增殖无显著作用，随活性炭浓度提高增殖系数有下降趋势；适量的活性炭和GA3可有效促进芽伸长。
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| 关 键 词： | 沼液 沼液 冬小麦 土壤质量 |
| 收稿时间： | 2011/10/9 0:00:00 |
| 修稿时间： | 2011/12/23 0:00:00 |
Study on the Tissue Culture of Pinus bungeana |
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| Zhang Li
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Yan Hui
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Gao Wenqiang
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Zhao Hongxia
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Fan Jinhui.Study on the Tissue Culture of Pinus bungeana[J].Chinese Agricultural Science Bulletin,2012,28(7):13-16. |
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| Authors: | Zhang Li Yan Hui Gao Wenqiang Zhao Hongxia Fan Jinhui |
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| Institution: | ( College of Forestry, Shandong Agricultural University , Tai ＇ an Shandong 271018) |
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| Abstract: | The aim was to establish efficient regeneration system. Mature embryo and cotyledon of Pinus bungeana were used as explants, after primary culture and propagation culture, the role of different plant growth regulator type and concentration, activated carbon, GA 3 in the various stages of tissue culture were studied. The results showed that the best initiated medium for cotyledon was MS ＋ 6-BA 3 mg/L ＋ NAA 0.1 mg/L＋TDZ 0.01 mg/L, the best initiated medium for mature embryo was MS＋6-BA 2 mg/L＋NAA 0.1 mg/L. The optimum shoot proliferation medium was MS＋NAA 0.05 mg/L. Pre-embryo （removal of hypocotyl） as explants, the optimum shoots rooting medium was 1/2MS＋IBA 0.3 mg/L. Activated carbon could not promote shoot proliferation, growth coefficient decrease with the increase of carbon concentration. Right amount of activated carbon and GA 3 effectively promoted the growth of buds. |
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| Keywords: | Pinus bungeana mature embryo cotyledon tissue culture |
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