| 河水中噻菌灵和敌百虫的SPE-RP-HPLC分析 |
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| 引用本文： | 高智席,吴艳红,黎司,胡明华,牟青松,曾启华.河水中噻菌灵和敌百虫的SPE-RP-HPLC分析[J].中国农学通报,2012,28(6):217-221. |
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| 作者姓名： | 高智席 吴艳红 黎司 胡明华 牟青松 曾启华 |
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| 作者单位： | 1. 遵义师范学院化学系,贵州遵义,563002
2. 重庆大学/三峡库区生态环境教育部重点实验室,重庆,400030 |
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| 基金项目： | 贵州省教育厅自然科学项目“赤水河流域水体及沉积物中环境激素分析调查和水环境风险评价研究”（[2010]085号）; 贵州省科技厅社会发展攻关项目“酸性废水治理回收的关键技术研究及应用”（黔科合SY字[2009]3050）; 遵义市科技局自然科学项目“没食子酸衍生物的开发与应用研究”（[2011]11号） |
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| 摘 要： | 为了对河水中噻菌灵和敌百虫残留进行高效的富集纯化和测定，建立了噻菌灵和敌百虫固相萃取-反相高效液相色谱法进行检测。河水中的噻菌灵和敌百虫用3 mol/L Empore 6 mL C18固相萃取小柱进行固相萃取。以Shim-pack VP-ODS 150 mm × 4.6 mm柱为分析柱，优化得到高效液相色谱分离条件：流动相为乙腈:水=75:25(V/V)，流速为0.8 mL/min，进样量20 μL。结果表明，噻菌灵和敌百虫在0.13~97.92 μg/mL (r=0.9961)、0.02~50 μg/mL (r=0.9994)浓度范围内与峰面积成良好线性关系,检出限分别为0.09 μg/mL、0.01 μg/mL，噻菌灵和敌百虫的回收率为87.6%~101.3%，相对标准偏差为1.7%~2.77%。可以看出，该方法操作简便快速，可作为河水中噻菌灵和敌百虫含量监测的控制方法。
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| 关 键 词： | 测度 测度 |
| 收稿时间： | 2011/10/28 0:00:00 |
| 修稿时间： | 2011/12/3 0:00:00 |
Analysis of Thiabendazole and Trichlorfon in River Water by RP-HPLC with Solid-phase Extraction |
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| Gao Zhixi
,
Wu Yanhong
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Li Si
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Hu Minghua
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Mu Qingsong
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Zeng Qihua.Analysis of Thiabendazole and Trichlorfon in River Water by RP-HPLC with Solid-phase Extraction[J].Chinese Agricultural Science Bulletin,2012,28(6):217-221. |
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| Authors: | Gao Zhixi Wu Yanhong Li Si Hu Minghua Mu Qingsong Zeng Qihua |
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| Institution: | 1Chemistry Department of Zunyi Normal College,Zunyi Guizhou 563002;2Key Laboratory of the Three Gorges Reservoir Region＇s Eco-environment,Ministry of Education/Chongqing University,Chongqing 400030) |
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| Abstract: | For better enrichment and purity of thiabendazole and trichlorfon in river water,a reversed-phase high performance liquid chromatograghic method with solid-phase extraction was used for the residue dynamic analysis of thiabendazole and trichlorfon.Water was subjected to solid-phase extraction（SPE） using 3 mol/L Empore 6 mL C18.The analytical column of HPLC was Shim-pack VP-ODS 150 mm × 4.6 mm with a mobile phase of CH3CN：H2O=75：25（V/V）,flow rate 0.8 mL/min,injection volume 20 μL.The SPE-HPLC method showed good linearity of thiabendazole and trichlorfon was obtained over the range of 0.13-97.92 μg/mL（r= 0.9961）,0.02-50 μg/mL（r=0.9994）.The detection limits were 0.09 μg/mL and 0.01 μg/mL for thiabendazole and trichlorfon.The average recoveries ranged from 87.6%-101.3% with relative standard deviation 1.7%-2.77%.In conclusion,the method was fast and simple for the residue detection and analysis of thiabendazole and trichlorfon in river water. |
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| Keywords: | river water solid-phase extraction trichlorfon thiabendazole RP-HPLC |
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