| 4个梅花品种的胚培养及愈伤组织诱导研究 |
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| 引用本文： | 石文芳,郝瑞杰,王史琴,张启翔.4个梅花品种的胚培养及愈伤组织诱导研究[J].中国农学通报,2012,28(10):198-201. |
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| 作者姓名： | 石文芳 郝瑞杰 王史琴 张启翔 |
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| 作者单位： | 北京林业大学园林学院/国家花卉工程中心,北京,100083 |
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| 基金项目： | 国家林业局林业公益项目“梅花新品种选育及产业化升级关键技术研究”（201004012）; 北京市教育委员会共建项目“梅花远缘杂交新品种选育及核心种质的保存繁殖与示范” |
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| 摘 要： | 为保存和扩繁梅花种质资源,筛选4个梅花品种,取胚进行胚培养,旨在建立梅花胚培养的无菌体系。以梅花(Prunus mume Sieb.Et Zucc.)天然杂交的未成熟胚为外植体,初步探索‘淡寒红’、‘南京红须’、‘双碧垂枝’、‘粉瓣’等梅花品种的胚培养体系,并在此基础上,利用叶片建立了高效的愈伤组织诱导体系。结果表明:胚培养的最适培养基为MS+6-BA 1.0 mg/L+IBA 0.2 mg/L,叶片愈伤组织诱导培养基为MS+2,4-D 1.0 mg/L,最适的叶龄为幼叶时期。梅花的胚培养中,不同品种的萌发率具有一定差异,难易顺序是单瓣品种群<垂枝品种群<朱砂品种群。
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| 关 键 词： | 梅花 胚培养 愈伤组织 |
| 收稿时间： | 2011/11/25 0:00:00 |
| 修稿时间： | 2012/2/13 0:00:00 |
Research on Embryo Culture and Callus Culture of Prunus mume Cultivars |
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| Shi Wenfang
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Hao Ruijie
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Wang Shiqin
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Zhang Qixiang.Research on Embryo Culture and Callus Culture of Prunus mume Cultivars[J].Chinese Agricultural Science Bulletin,2012,28(10):198-201. |
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| Authors: | Shi Wenfang Hao Ruijie Wang Shiqin Zhang Qixiang |
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| Institution: | ( College of Landscape Architecture,Beijing Forestry University / National Flower Engineering Centre ,Beijing 100083) |
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| Abstract: | In order to conserve and multiply germplasm rescources of Mei flower,the gern-free system of embryo-culture was constructed in seeds of four cultivars.The contents of this paper were as follows：the author conducted immature embryos culture of Prunus mume ＇Danhanhong＇ , ＇Nanjing Hongxu＇ , ＇Shuangbi Chuizhi＇ , ＇Fenban＇ derived from pollination.In addition,using leaf of seeding cultivation as explants,the efficient of callus culture was conducted.Results showed that the perfect culture medium was MS ＋ 6-BA 1.0 mg/L＋IBA 0.2 mg/L.The optimum callus culture was MS＋2,4-D 1.0 mg/L and perfect leafage was young period.Germination rate of cultivars was different.The difficulty was single-lobe Mei Branchhanger wood Mei Branchcinnabar Mei Branch. |
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| Keywords: | Prunus mume embryo culture callus culture |
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