| 辅助营养源对蜜环菌栽培种的影响 |
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| 引用本文： | 敖新宇,彭述敏,陈玉惠.辅助营养源对蜜环菌栽培种的影响[J].中国农学通报,2012,28(7):212-216. |
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| 作者姓名： | 敖新宇 彭述敏 陈玉惠 |
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| 作者单位： | 1. 西南林业大学生命科学学院,昆明,650224
2. 西南林业大学林学院,昆明,650224 |
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| 基金项目： | 云南省森林保护学重点学科（XKZ200905） |
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| 摘 要： | 以昭通本地的一株优良蜜环菌SNA04为研究对象，在蜜环菌栽培种制作过程中添加一定量的不同辅助营养物质以考察辅助营养物质对蜜环菌菌株生长的影响，为实现昭通本地蜜环菌的快速工业化制种及大规模生产奠定基础。用SNA04作菌种，栎树枝条作主要培养基，不同浓度梯度的葡萄糖、乙醇、酵母膏、蛋白胨和土豆汁作辅助营养源培养蜜环菌栽培种。与对照相比，加入辅助营养源后，菌索生长速度提高，菌株生长状况得到改良，表现为菌索长势旺、分枝多、色白、粗壮等。其中，菌索在加有250 g/L土豆汁菌种瓶中长势最强，平均生长速度达到了1.29 cm/天，比对照中的菌索生长速度快0.48 cm/天。所添加的5种营养液培养基中，最适合菌索生长的是加有土豆汁的菌枝培养基。土豆是昭通地区主产作物，具有较高产量，可以将其作为蜜环菌栽培种的辅助营养源加以利用。
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| 关 键 词： | 品种 品种 免疫应激 氨基酸消化率 |
| 收稿时间： | 2011/9/18 0:00:00 |
| 修稿时间： | 2011/11/23 0:00:00 |
Effect of Supplementary Nutrient Sources on the Growth of the Cultivation Seeds of Armillaria mellea |
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| Ao Xinyu
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Peng Shumin
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Chen Yuhui.Effect of Supplementary Nutrient Sources on the Growth of the Cultivation Seeds of Armillaria mellea[J].Chinese Agricultural Science Bulletin,2012,28(7):212-216. |
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| Authors: | Ao Xinyu Peng Shumin Chen Yuhui |
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| Institution: | 1 Faculty of Life Science, Southwest Forestry University , Kunming 650224; 2 Faculty of Forestry, Southwest Forestry University , Kunming 650224) |
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| Abstract: | Taking SNA04, a good strain of A. mellea, as objective, certain different supplementary nutrient sources were added in the course of culturing the A. mellea so as to observe the effect of the supplementary nutrient sources on the growth of the A. mellea, which would lay the foundation for the seed-producing industrialization and mass production of the A. mellea in Zhaotong Prefecture. By using SNA04 as strain of the A. mellea and oak branches as main medium, different concentration gradients of glucose, ethanol, yeast extracts, protein peptone and potato juice were used as the supplementary nutrient sources to cultivate cultivation seeds of A. mellea. The results were as follows： the growth of rhizomorphs after adding the supplementary nutrient sources, compared with the control, accelerated and the growth of strains improved, manifesting as the rhizomrphs of high-speed growth, multiple branching and white complexion as well as robustness. In particular, the growth speed of rhizomorphs was the fastest in the strain bottles with the potato juice of 250 g/L reaching an average growth speed of 1.29 cm/d, which boosted the strains of the control 0.48 cm/d faster. Among the five sorts of nutrient sources added, the fittest for the growth of the rhizomrphs was the sources added in potatoes. Potato was the main products of high-yielding crop in Zhaotong Prefecture, which would be used as supplementary nutrient source to cultivate the cultivation seeds of A. mellea. |
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| Keywords: | Gastrodia elata B1 Armillaria mellea supplementary nutrient source |
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