| 培养基组分对纤维杆菌X4-9发酵产纤维素酶的影响 |
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| 引用本文： | 叶川,李昆太,黄欠如,钟义军,武琳,成艳红.培养基组分对纤维杆菌X4-9发酵产纤维素酶的影响[J].中国农学通报,2012,28(6):166-172. |
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| 作者姓名： | 叶川 李昆太 黄欠如 钟义军 武琳 成艳红 |
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| 作者单位： | 1. 江西省红壤研究所,江西进贤331717;农业部鄱阳湖区红壤耕地质量与环境重点野外科学观测试验站,江西进贤331717
2. 江西农业大学,南昌,330045 |
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| 基金项目： | “十一五”国家科技支撑课题“赣东北低丘岗地区红壤退化防治与生态农业技术集成和示范”（2009BADC6B04） |
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| 摘 要： | 考察发酵培养基组分对纤维杆菌X4-9发酵产葡聚糖内切酶、葡聚糖外切酶和β-葡萄糖苷酶的影响。应用DPS软件的二次回归旋转中心组合实验设计方法，对纤维杆菌X4-9的发酵培养基（花生秸杆粉、蔗糖、尿素、(NH4)2SO4、硫酸镁、磷酸二氢钾）进行了优化。结果表明：在试验数值范围内，花生秸杆粉、蔗糖和(NH4)2SO4对酶活均会产生极显著的正效应，而尿素、硫酸镁和磷酸二氢钾的用量则具有明显的负效应；在优化的发酵培养基配方（花生秸杆粉30 g/L、蔗糖20 g/L、尿素2.5 g/L、(NH4)2SO4 10 g/L）下，葡萄糖内切酶、葡聚糖外切酶和β-葡萄糖苷酶的酶活分别达到2.705163，5.723014和3.614921 I U/mL，比初始发酵培养基配方下的各酶活分别提高了123.66%，146.32%和186.40%。纤维杆菌X4-9均能同时产生葡萄糖内切酶、葡聚糖外切酶、β-葡萄糖苷酶等纤维素降解复合酶系，且这些酶高产的培养基条件较为一致。
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| 关 键 词： | 遗传结构 遗传结构 |
| 收稿时间： | 2011/7/20 0:00:00 |
| 修稿时间： | 9/8/2011 12:00:00 AM |
Effects of the Fermentation Medium Components on the Cellulase Production by Cellulomonas X4-9 |
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| Ye Chuan
,
Li Kuntai
,
Huang Qianru
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Zhong Yijun
,
Wu Lin
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Cheng Yanhong.Effects of the Fermentation Medium Components on the Cellulase Production by Cellulomonas X4-9[J].Chinese Agricultural Science Bulletin,2012,28(6):166-172. |
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| Authors: | Ye Chuan Li Kuntai Huang Qianru Zhong Yijun Wu Lin Cheng Yanhong |
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| Institution: | 1Red Soil Research Institute of Jiangxi Province,Jinxian Jiangxi 331717;2Testing Station of Red Soil Cultivation Land Quality and Environment in Poyang Lake Region of the Ministry of Agriculture of China,Jinxian Jiangxi 331717;3Jiangxi Agriculture University,Nanchang 330045) |
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| Abstract: | In this paper,the effects of the components in fermentation medium on the endoglucanase,exoglucanase and β-glucosidase producing by Cellulomonas X4-9 were investigated.The rotational quadratic orthogonal experiment design by DPS soft was used to optimize the fermentation medium（peanut straw powder,sucrose,carbamide,（NH4）2SO4,MgSO4 and KH2PO4） of Cellulomonas X4-9.The results showed that peanut straw powder,sucrose and（NH4）2SO4 had a significantly positive effect on the biosynthesis of the above enzymes,but carbamide,MgSO4 and KH2PO4 caused a negative effect.In the case of the optimal fermentation medium（Peanut straw powder 30 g/L,sucrose 20 g/L,carbamide 2.5 g/L,（NH4）2SO4 10 g/L） for Cellulomonas X4-9,the final activity of endoglucanase,eXoglucanase and β-glucosidase was 2.705163,5.723014,3.614921 IU/mL,which was 123.66%,146.32% and 186.40% higher than that obtained under the original medium recipe,respectively.Cellulomonas X4-9 could produce a group of enzymes including endoglucanase,exoglucanase and β-glucosidase,and the condition of fermentation medium was coincident to the higher productivity. |
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| Keywords: | Cellulomonas X4-9 endoglucanase exoglucanase β-glucosidase fermentation medium |
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