| 高效降解棉酚菌株的筛选鉴定及毒性试验 |
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| 引用本文： | 张丛,李佳,袁洪水,郝志敏,朱宝成.高效降解棉酚菌株的筛选鉴定及毒性试验[J].中国农学通报,2012,28(12):112-117. |
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| 作者姓名： | 张丛 李佳 袁洪水 郝志敏 朱宝成 |
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| 作者单位： | 河北农业大学生命科学学院微生物与生化药学系,河北保定,071001 |
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| 摘 要： | 为了筛选一株能高效降解棉籽饼中棉酚毒素的菌株，以棉酚为唯一碳源，从棉田土壤中分离筛选对棉酚具有降解作用的菌株，得到具有较高降解活性的菌株M-9，其降解率达到94.74%，对其进行了菌株形态观察和生理生化鉴定并结合其16S rDNA序列，初步确定其为枯草芽孢杆菌(Bacillus subtilis)。通过对M-9菌株的脱毒效果研究表明，其具有降解棉籽饼中游离棉酚的能力，脱毒后棉籽饼中游离棉酚的含量降至33.18 mg/kg，符合中国国家标准饲料卫生标准规定(GB13078-2001)：饲料原料棉籽饼中游离棉酚含量≤1200 mg/kg。并进行毒性饲喂试验，从而确定菌株M-9处理后的棉籽饼粉对小白鼠无毒性。
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| 关 键 词： | 田园城市 田园城市 |
| 收稿时间： | 2011/10/25 0:00:00 |
| 修稿时间： | 2011/12/23 0:00:00 |
Isolation and Identification of Strains for Digesting Free-gossypol and Toxicity Test |
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| Zhang Cong
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Li Jia
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Yuan Hongshui
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Hao Zhimin
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Zhu Baocheng.Isolation and Identification of Strains for Digesting Free-gossypol and Toxicity Test[J].Chinese Agricultural Science Bulletin,2012,28(12):112-117. |
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| Authors: | Zhang Cong Li Jia Yuan Hongshui Hao Zhimin Zhu Baocheng |
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| Institution: | (Agricultural University of Hebei College of Life Sciences, Baoding Hebei 071001) |
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| Abstract: | To obtain high efficient gossypol-degraded bacteria, a strain named M-9 was isolated from a soil microcosm by used the gossypol as the only carbon resource and investigated due to the finding that it could degrade free-gossypol. After observe its morphology, analyzed its biochemical characteristic and combine with 16S rDNA sequence, it was identified as the Bacillus subtilis preliminarily. The detoxification research showed that, the M-9 bacterial strain had the ability to be degraded the free gossypol in the cottonseed cake, and the rate of degradation was 94.74% , residual quantity was up to our national feed hygiene standard （GB13078-2001）, the free gossypol content of the feedstuff cottonseed cake was no more than 1200 mg/kg. And via toxicity feeding test, we make sure that, the treatment to the bacterial strain M-9 had reduced the toxicity of gossypol. |
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| Keywords: | gossypol degradation strains cottonseed cake screening safety biological detoxification |
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